7y8p: Difference between revisions
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==Crystal structure of 4'-selenoRNA duplex== | |||
<StructureSection load='7y8p' size='340' side='right'caption='[[7y8p]], [[Resolution|resolution]] 1.50Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7y8p]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Y8P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Y8P FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IKS:[(2~{R},3~{S},4~{R},5~{R})-5-(4-azanyl-2-oxidanylidene-pyrimidin-1-yl)-3,4-bis(oxidanyl)selenolan-2-yl]methyl+dihydrogen+phosphate'>IKS</scene>, <scene name='pdbligand=ILK:[(2~{R},3~{S},4~{R},5~{R})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)selenolan-2-yl]methyl+dihydrogen+phosphate'>ILK</scene>, <scene name='pdbligand=NCO:COBALT+HEXAMMINE(III)'>NCO</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7y8p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7y8p OCA], [https://pdbe.org/7y8p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7y8p RCSB], [https://www.ebi.ac.uk/pdbsum/7y8p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7y8p ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
A large number of chemically modified oligonucleotides (ONs) have been developed for RNA-based technologies. In most modified RNAs, the characteristic 2'-hydroxyl (2'-OH) groups are removed to enhance both nuclease resistance and hybridization ability. However, the importance of the 2'-OH group in RNA structure and function is well known. Here, we report the synthesis and properties of 4'-selenoRNA in which all four nucleoside units retain the 2'-OH groups but contain a selenium atom instead of an oxygen atom at the 4'-position of the furanose ring. 4'-SelenoRNA has enhanced ability to form duplexes with RNA, and high endonuclease resistance despite the presence of the 2'-OH groups. X-ray crystallography analysis showed that the 4'-selenoRNA duplex adopts an A-conformation, similar to natural RNA, although one 4'-selenocytidine residue has unusual South-type sugar puckering. Furthermore, preliminary studies using 4'-seleno-modified siRNAs suggest that 4'-selenoRNA may be applicable to RNA interference technology. Collectively, our results raise the possibility of a new class of modified RNA in which 2'-OH groups do not need to be masked. | |||
Synthesis and properties of fully-modified 4'-selenoRNA, an endonuclease-resistant RNA analog.,Ota M, Takahashi H, Nogi Y, Kagotani Y, Saito-Tarashima N, Kondo J, Minakawa N Bioorg Med Chem. 2022 Dec 15;76:117093. doi: 10.1016/j.bmc.2022.117093. Epub 2022 , Nov 19. PMID:36434923<ref>PMID:36434923</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 7y8p" style="background-color:#fffaf0;"></div> | ||
[[Category: Kondo | == References == | ||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Synthetic construct]] | |||
[[Category: Kondo J]] | |||
[[Category: Minakawa N]] | |||
[[Category: Ohta M]] | |||
[[Category: Takahashi H]] | |||
[[Category: Tarashima N]] | |||
Revision as of 07:26, 3 May 2023
Crystal structure of 4'-selenoRNA duplex
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