4yc7: Difference between revisions

Revision as of 08:08, 3 May 2023

Crystal structure of human FMNL2 GBD-FH3 Domains bound to Cdc42-GppNHp

Structural highlights

4yc7 is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	GNP, MG
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

FMNL2_HUMAN Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the cortical actin filament dynamics.^[1]

Publication Abstract from PubMed

Formins are actin polymerization factors that elongate unbranched actin filaments at the barbed end. Rho family GTPases activate Diaphanous-related formins through the relief of an autoregulatory interaction. The crystal structures of the N-terminal domains of human FMNL1 and FMNL2 in complex with active Cdc42 show that Cdc42 mediates contacts with all five armadillo repeats of the formin with specific interactions formed by the Rho-GTPase insert helix. Mutation of three residues within Rac1 results in a gain-of-function mutation for FMNL2 binding and reconstitution of the Cdc42 phenotype in vivo. Dimerization of FMNL1 through a parallel coiled coil segment leads to formation of an umbrella-shaped structure that-together with Cdc42-spans more than 15 nm in diameter. The two interacting FMNL-Cdc42 heterodimers expose six membrane interaction motifs on a convex protein surface, the assembly of which may facilitate actin filament elongation at the leading edge of lamellipodia and filopodia.

The structure of FMNL2-Cdc42 yields insights into the mechanism of lamellipodia and filopodia formation.,Kuhn S, Erdmann C, Kage F, Block J, Schwenkmezger L, Steffen A, Rottner K, Geyer M Nat Commun. 2015 May 12;6:7088. doi: 10.1038/ncomms8088. PMID:25963737^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Bai SW, Herrera-Abreu MT, Rohn JL, Racine V, Tajadura V, Suryavanshi N, Bechtel S, Wiemann S, Baum B, Ridley AJ. Identification and characterization of a set of conserved and new regulators of cytoskeletal organization, cell morphology and migration. BMC Biol. 2011 Aug 11;9:54. doi: 10.1186/1741-7007-9-54. PMID:21834987 doi:10.1186/1741-7007-9-54
↑ Kuhn S, Erdmann C, Kage F, Block J, Schwenkmezger L, Steffen A, Rottner K, Geyer M. The structure of FMNL2-Cdc42 yields insights into the mechanism of lamellipodia and filopodia formation. Nat Commun. 2015 May 12;6:7088. doi: 10.1038/ncomms8088. PMID:25963737 doi:https://dx.doi.org/10.1038/ncomms8088

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OCA

[1] Bai SW, Herrera-Abreu MT, Rohn JL, Racine V, Tajadura V, Suryavanshi N, Bechtel S, Wiemann S, Baum B, Ridley AJ. Identification and characterization of a set of conserved and new regulators of cytoskeletal organization, cell morphology and migration. BMC Biol. 2011 Aug 11;9:54. doi: 10.1186/1741-7007-9-54. PMID:21834987 doi:10.1186/1741-7007-9-54

[2] Kuhn S, Erdmann C, Kage F, Block J, Schwenkmezger L, Steffen A, Rottner K, Geyer M. The structure of FMNL2-Cdc42 yields insights into the mechanism of lamellipodia and filopodia formation. Nat Commun. 2015 May 12;6:7088. doi: 10.1038/ncomms8088. PMID:25963737 doi:https://dx.doi.org/10.1038/ncomms8088

[1]

[2]

@@ Line 3: / Line 3: @@
 <StructureSection load='4yc7' size='340' side='right'caption='[[4yc7]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4yc7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YC7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4YC7 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4yc7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YC7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YC7 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">FMNL2, FHOD2, KIAA1902 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), CDC42 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4yc7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yc7 OCA], [https://pdbe.org/4yc7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4yc7 RCSB], [https://www.ebi.ac.uk/pdbsum/4yc7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4yc7 ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4yc7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yc7 OCA], [http://pdbe.org/4yc7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4yc7 RCSB], [http://www.ebi.ac.uk/pdbsum/4yc7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4yc7 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/FMNL2_HUMAN FMNL2_HUMAN]] Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the cortical actin filament dynamics.<ref>PMID:21834987</ref>  [[http://www.uniprot.org/uniprot/CDC42_HUMAN CDC42_HUMAN]] Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. Plays a role in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. Mediates CDC42-dependent cell migration.<ref>PMID:14978216</ref> <ref>PMID:15642749</ref> <ref>PMID:17038317</ref>
+[https://www.uniprot.org/uniprot/FMNL2_HUMAN FMNL2_HUMAN] Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the cortical actin filament dynamics.<ref>PMID:21834987</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
 [[Category: Large Structures]]
-[[Category: Geyer, M]]
+[[Category: Geyer M]]
-[[Category: Kuhn, S]]
+[[Category: Kuhn S]]
-[[Category: Armadillo repeat]]
-[[Category: Cell cycle]]
-[[Category: Rho gtpase]]
-[[Category: Signaling protein]]

4yc7: Difference between revisions

Revision as of 08:08, 3 May 2023

Crystal structure of human FMNL2 GBD-FH3 Domains bound to Cdc42-GppNHp

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

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