4yu5: Difference between revisions

Revision as of 07:25, 10 May 2023

Crystal structure of selenomethionine variant of Bacillus anthracis immune inhibitor A2 peptidase zymogen

Structural highlights

4yu5 is a 2 chain structure with sequence from Bacillus cereus biovar anthracis str. CI. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	CA, GOL, K, KH2, MSE, ZN
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

D8H130_BACAI

Publication Abstract from PubMed

Immune inhibitor A(InhA)-type metallopeptidases are potential virulence factors secreted by members of the Bacillus cereus group. Two paralogs from anthrax-causing Bacillus anthracis (BaInhA1 and BaInhA2) were shown to degrade host tissue proteins with broad substrate specificity. Analysis of their activation mechanism and the crystal structure of a zymogenic BaInhA2 variant revealed a approximately 750-residue four-domain structure featuring a pro-peptide, a catalytic domain, a domain reminiscent of viral envelope glycoproteins, and a MAM domain grafted into the latter. This domain, previously found only in eukaryotes, is required for proper protein expression in B. anthracis and evinces certain flexibility. Latency is uniquely modulated by the N-terminal segment of the pro-peptide, which binds the catalytic zinc through its alpha-amino group and occupies the primed side of the active-site cleft. The present results further our understanding of the modus operandi of an anthrax secretome regulator.

Structural Basis for Latency and Function of Immune Inhibitor A Metallopeptidase, a Modulator of the Bacillus anthracis Secretome.,Arolas JL, Goulas T, Pomerantsev AP, Leppla SH, Gomis-Ruth FX Structure. 2016 Jan 5;24(1):25-36. doi: 10.1016/j.str.2015.10.015. PMID:26745529^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Arolas JL, Goulas T, Pomerantsev AP, Leppla SH, Gomis-Ruth FX. Structural Basis for Latency and Function of Immune Inhibitor A Metallopeptidase, a Modulator of the Bacillus anthracis Secretome. Structure. 2016 Jan 5;24(1):25-36. doi: 10.1016/j.str.2015.10.015. PMID:26745529 doi:https://dx.doi.org/10.1016/j.str.2015.10.015

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OCA

[1] Arolas JL, Goulas T, Pomerantsev AP, Leppla SH, Gomis-Ruth FX. Structural Basis for Latency and Function of Immune Inhibitor A Metallopeptidase, a Modulator of the Bacillus anthracis Secretome. Structure. 2016 Jan 5;24(1):25-36. doi: 10.1016/j.str.2015.10.015. PMID:26745529 doi:https://dx.doi.org/10.1016/j.str.2015.10.015

[1]

@@ Line 3: / Line 3: @@
 <StructureSection load='4yu5' size='340' side='right'caption='[[4yu5]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4yu5]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YU5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4YU5 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4yu5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_cereus_biovar_anthracis_str._CI Bacillus cereus biovar anthracis str. CI]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YU5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YU5 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=KH2:3-(1-METHYLPIPERIDINIUM-1-YL)PROPANE-1-SULFONATE'>KH2</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=KH2:3-(1-METHYLPIPERIDINIUM-1-YL)PROPANE-1-SULFONATE'>KH2</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4yu5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yu5 OCA], [https://pdbe.org/4yu5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4yu5 RCSB], [https://www.ebi.ac.uk/pdbsum/4yu5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4yu5 ProSAT]</span></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4yu6|4yu6]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4yu5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yu5 OCA], [http://pdbe.org/4yu5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4yu5 RCSB], [http://www.ebi.ac.uk/pdbsum/4yu5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4yu5 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/D8H130_BACAI D8H130_BACAI]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 22: @@
 __TOC__
 </StructureSection>
+[[Category: Bacillus cereus biovar anthracis str. CI]]
 [[Category: Large Structures]]
-[[Category: Arolas, J L]]
+[[Category: Arolas JL]]
-[[Category: Gomis-Ruth, F X]]
+[[Category: Gomis-Ruth FX]]
-[[Category: Goulas, T]]
+[[Category: Goulas T]]
-[[Category: Hydrolase]]
-[[Category: Metallopeptidase]]
-[[Category: Metzincin]]

4yu5: Difference between revisions

Revision as of 07:25, 10 May 2023

Crystal structure of selenomethionine variant of Bacillus anthracis immune inhibitor A2 peptidase zymogen

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

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