4z7k

Crystal structure of CRISPR RNA processing endoribonuclease Cas6b

spinqualitylabelsall models 4z7k, resolution 3.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4z7k is a 3 chain structure with sequence from Methanococcus maripaludis and Methanococcus maripaludis C5. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A4FXZ3_METM5 Publication Abstract from PubMed A subclass of recently discovered CRISPR repeat RNA in bacteria contains minimally recognizable structural features that facilitate an unknown mechanism of recognition and processing by the Cas6 family of endoribonucleases. Cocrystal structures of Cas6 from Methanococcus maripaludis (MmCas6b) bound with its repeat RNA revealed a dual site binding structure and a cleavage site conformation poised for phosphodiester bond breakage. Two non-interacting MmCas6b bind to two separate AAYAA motifs within the same repeat, one distal and one adjacent to the cleavage site. This bound structure potentially competes with a stable but non-productive RNA structure. At the cleavage site, MmCas6b supplies a base pair mimic to stabilize a short 2 base pair stem immediately upstream of the scissile phosphate. Complementary biochemical analyses support the dual-AAYAA binding model and a critical role of the protein-RNA base pair mimic. Our results reveal a previously unknown method of processing non-stem-loop CRISPR RNA by Cas6. A Non-Stem-Loop CRISPR RNA Is Processed by Dual Binding Cas6.,Shao Y, Richter H, Sun S, Sharma K, Urlaub H, Randau L, Li H Structure. 2016 Apr 5;24(4):547-554. doi: 10.1016/j.str.2016.02.009. Epub 2016, Mar 17. PMID:26996962 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also CRISPR subtype I-B

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