4z8a: Difference between revisions

Revision as of 07:43, 10 May 2023

SH3-III of Drosophila Rim-binding protein bound to a Cacophony derived peptide

Structural highlights

4z8a is a 2 chain structure with sequence from Drosophila melanogaster. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	ACE, NH2
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

A0A0B4JDC9_DROME

Publication Abstract from PubMed

Synaptic vesicles (SVs) fuse at active zones (AZs) covered by a protein scaffold, at Drosophila synapses comprised of ELKS family member Bruchpilot (BRP) and RIM-binding protein (RBP). We here demonstrate axonal co-transport of BRP and RBP using intravital live imaging, with both proteins co-accumulating in axonal aggregates of several transport mutants. RBP, via its C-terminal Src-homology 3 (SH3) domains, binds Aplip1/JIP1, a transport adaptor involved in kinesin-dependent SV transport. We show in atomic detail that RBP C-terminal SH3 domains bind a proline-rich (PxxP) motif of Aplip1/JIP1 with submicromolar affinity. Pointmutating this PxxP motif provoked formation of ectopic AZ-like structures at axonal membranes. Direct interactions between AZ proteins and transport adaptors seem to provide complex avidity and shield synaptic interaction surfaces of pre-assembled scaffold protein transport complexes, thus, favouring physiological synaptic AZ assembly over premature assembly at axonal membranes.

A high affinity RIM-binding protein/Aplip1 interaction prevents the formation of ectopic axonal active zones.,Siebert M, Bohme MA, Driller JH, Babikir H, Mampell MM, Rey U, Ramesh N, Matkovic T, Holton N, Reddy-Alla S, Gottfert F, Kamin D, Quentin C, Klinedinst S, Andlauer TF, Hell SW, Collins CA, Wahl MC, Loll B, Sigrist SJ Elife. 2015 Aug 14;4. doi: 10.7554/eLife.06935. PMID:26274777^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Siebert M, Bohme MA, Driller JH, Babikir H, Mampell MM, Rey U, Ramesh N, Matkovic T, Holton N, Reddy-Alla S, Gottfert F, Kamin D, Quentin C, Klinedinst S, Andlauer TF, Hell SW, Collins CA, Wahl MC, Loll B, Sigrist SJ. A high affinity RIM-binding protein/Aplip1 interaction prevents the formation of ectopic axonal active zones. Elife. 2015 Aug 14;4. doi: 10.7554/eLife.06935. PMID:26274777 doi:https://dx.doi.org/10.7554/eLife.06935

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Siebert M, Bohme MA, Driller JH, Babikir H, Mampell MM, Rey U, Ramesh N, Matkovic T, Holton N, Reddy-Alla S, Gottfert F, Kamin D, Quentin C, Klinedinst S, Andlauer TF, Hell SW, Collins CA, Wahl MC, Loll B, Sigrist SJ. A high affinity RIM-binding protein/Aplip1 interaction prevents the formation of ectopic axonal active zones. Elife. 2015 Aug 14;4. doi: 10.7554/eLife.06935. PMID:26274777 doi:https://dx.doi.org/10.7554/eLife.06935

[1]

@@ Line 3: / Line 3: @@
 <StructureSection load='4z8a' size='340' side='right'caption='[[4z8a]], [[Resolution|resolution]] 1.76&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4z8a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z8A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Z8A FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4z8a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z8A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Z8A FirstGlance]. <br>
-</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Rbp, Dmel_CG43073 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4z8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z8a OCA], [https://pdbe.org/4z8a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4z8a RCSB], [https://www.ebi.ac.uk/pdbsum/4z8a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4z8a ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4z8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z8a OCA], [http://pdbe.org/4z8a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4z8a RCSB], [http://www.ebi.ac.uk/pdbsum/4z8a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4z8a ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/CAC1A_DROME CAC1A_DROME]] Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, neurotransmitter release, gene expression, cell motility, cell division and cell death (By similarity). Probably encodes a dihydropyridine-insensitive current. Vital for survival to adulthood.<ref>PMID:8987815</ref> <ref>PMID:9649530</ref>
+[https://www.uniprot.org/uniprot/A0A0B4JDC9_DROME A0A0B4JDC9_DROME]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Drome]]
+[[Category: Drosophila melanogaster]]
 [[Category: Large Structures]]
-[[Category: Boehme, A M]]
+[[Category: Boehme AM]]
-[[Category: Driller, J H]]
+[[Category: Driller JH]]
-[[Category: Holton, N]]
+[[Category: Holton N]]
-[[Category: Loll, B]]
+[[Category: Loll B]]
-[[Category: Siebert, M]]
+[[Category: Siebert M]]
-[[Category: Sigrist, S J]]
+[[Category: Sigrist SJ]]
-[[Category: Wahl, M C]]
+[[Category: Wahl MC]]
-[[Category: Active zone]]
-[[Category: Cacophony peptide]]
-[[Category: Rim-binding protein]]
-[[Category: Sh3 domain]]
-[[Category: Synapse]]

4z8a: Difference between revisions

Revision as of 07:43, 10 May 2023

SH3-III of Drosophila Rim-binding protein bound to a Cacophony derived peptide

Structural highlights

Function

Publication Abstract from PubMed

References

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