7tv2: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==X-ray crystal structure of HIV-2 CA protein CTD== | |||
<StructureSection load='7tv2' size='340' side='right'caption='[[7tv2]], [[Resolution|resolution]] 1.98Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7tv2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_immunodeficiency_virus_2 Human immunodeficiency virus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TV2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7TV2 FirstGlance]. <br> | |||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7tv2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7tv2 OCA], [https://pdbe.org/7tv2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7tv2 RCSB], [https://www.ebi.ac.uk/pdbsum/7tv2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7tv2 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/A0A7D6CKX3_9HIV2 A0A7D6CKX3_9HIV2] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Retrovirus immature particle morphology consists of a membrane enclosed, pleomorphic, spherical and incomplete lattice of Gag hexamers. Previously, we demonstrated that human immunodeficiency virus type 2 (HIV-2) immature particles possess a distinct and extensive Gag lattice morphology. To better understand the nature of the continuously curved hexagonal Gag lattice, we have used single particle cryo-electron microscopy with a retrovirus to determine the HIV-2 Gag lattice structure for immature virions. The reconstruction map at 5.5 A resolution revealed a stable, wineglass-shaped Gag hexamer structure with structural features consistent with other lentiviral immature Gag structures. Cryo-electron tomography provided evidence for nearly complete ordered Gag lattice structures in HIV-2 immature particles. We also solved a 1.98 A resolution crystal structure of the carboxyl-terminal domain (CTD) of the HIV-2 capsid (CA) protein that identified a structured helix 12 supported via an interaction of helix 10 in the absence of the SP1 region of Gag. Residues at the helix 10-12 interface proved critical in maintaining HIV-2 particle release and infectivity. Taken together, our findings provide the first 3D organization of HIV-2 immature Gag lattice and important insights into both HIV Gag lattice stabilization and virus maturation. | |||
HIV-2 Immature Particle Morphology Provides Insights into Gag Lattice Stability and Virus Maturation.,Talledge N, Yang H, Shi K, Coray R, Yu G, Arndt WG, Meng S, Baxter GC, Mendonca LM, Castano-Diez D, Aihara H, Mansky LM, Zhang W J Mol Biol. 2023 May 5:168143. doi: 10.1016/j.jmb.2023.168143. PMID:37150290<ref>PMID:37150290</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 7tv2" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Human immunodeficiency virus 2]] | |||
[[Category: Large Structures]] | |||
[[Category: Aihara H]] | |||
[[Category: Shi K]] | |||
Revision as of 06:55, 18 May 2023
X-ray crystal structure of HIV-2 CA protein CTD
| ||||||||||||
