6de6: Difference between revisions

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<StructureSection load='6de6' size='340' side='right'caption='[[6de6]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
<StructureSection load='6de6' size='340' side='right'caption='[[6de6]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6de6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhizobium_sp._4-9 Rhizobium sp. 4-9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DE6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DE6 FirstGlance]. <br>
<table><tr><td colspan='2'>[[6de6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhizobium_sp._4-9 Rhizobium sp. 4-9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DE6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6DE6 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6de6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6de6 OCA], [http://pdbe.org/6de6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6de6 RCSB], [http://www.ebi.ac.uk/pdbsum/6de6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6de6 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6de6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6de6 OCA], [https://pdbe.org/6de6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6de6 RCSB], [https://www.ebi.ac.uk/pdbsum/6de6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6de6 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q60I59_9HYPH Q60I59_9HYPH]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Histamine dehydrogenase from the gram-negative bacterium Rhizobium sp. 4-9 (HaDHR) is a member of a small family of dehydrogenases containing a covalently attached FMN, and the only member so far identified to date that does not exhibit substrate inhibition. In this study, we present the 2.1 A resolution crystal structure of HaDHR. This new structure allowed for the identification of the internal electron transfer pathway to abiological ferrocene-based mediators. Alanine 437 was identified as the exit point of electrons from the Fe(4)S(4) cluster. The enzyme was modified with a Ser436Cys mutation to facilitate covalent attachment of a ferrocene moiety. When modified with Fc-maleimide, this new construct demonstrated direct electron transfer from the enzyme to a gold electrode in a histamine concentration-dependent manner without the need for any additional electron mediators.
Structure of Rhizobium sp. 4-9 histamine dehydrogenase and analysis of the electron transfer pathway to an abiological electron acceptor.,Goyal P, Deay D 3rd, Seibold S, Candido ACL, Lovell S, Battaile KP, Wilson GS, Richter ML, Petillo PA Arch Biochem Biophys. 2023 May 3;742:109612. doi: 10.1016/j.abb.2023.109612. PMID:37146865<ref>PMID:37146865</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6de6" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Rhizobium sp. 4-9]]
[[Category: Rhizobium sp. 4-9]]
[[Category: Goyal, P]]
[[Category: Goyal P]]
[[Category: Lovell, S]]
[[Category: Lovell S]]
[[Category: Richter, M]]
[[Category: Richter M]]
[[Category: 4fe-4s cluster]]
[[Category: Fmn binding]]
[[Category: Histamine dehydrogenase]]
[[Category: Oxidoreductase]]
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