8b2w: Difference between revisions

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'''Unreleased structure'''


The entry 8b2w is ON HOLD  until Paper Publication
==Millisecond cryo-trapping by the spitrobot crystal plunger, CTX-M-14 E166A, Ampicillin, 500 MS==
<StructureSection load='8b2w' size='340' side='right'caption='[[8b2w]], [[Resolution|resolution]] 1.78&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8b2w]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8B2W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8B2W FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AIX:(2R,4S)-2-[(1R)-1-{[(2R)-2-AMINO-2-PHENYLACETYL]AMINO}-2-OXOETHYL]-5,5-DIMETHYL-1,3-THIAZOLIDINE-4-CARBOXYLIC+ACID'>AIX</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8b2w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8b2w OCA], [https://pdbe.org/8b2w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8b2w RCSB], [https://www.ebi.ac.uk/pdbsum/8b2w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8b2w ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/D2D9A0_KLEPN D2D9A0_KLEPN]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
We introduce the spitrobot, a protein crystal plunger, enabling reaction quenching via cryo-trapping with a time-resolution in the millisecond range. Protein crystals are mounted on canonical micromeshes on an electropneumatic piston, where the crystals are kept in a humidity and temperature-controlled environment, then reactions are initiated via the liquid application method (LAMA) and plunging into liquid nitrogen is initiated after an electronically set delay time to cryo-trap intermediate states. High-magnification images are automatically recorded before and after droplet deposition, prior to plunging. The SPINE-standard sample holder is directly plunged into a storage puck, enabling compatibility with high-throughput infrastructure. Here we demonstrate binding of glucose and 2,3-butanediol in microcrystals of xylose isomerase, and of avibactam and ampicillin in microcrystals of the extended spectrum beta-lactamase CTX-M-14. We also trap reaction intermediates and conformational changes in macroscopic crystals of tryptophan synthase to demonstrate that the spitrobot enables insight into catalytic events.


Authors: Mehrabi, P., Sung, S., von Stetten, D., Prester, A., Hatton, C.E., Kleine-Doepke, S., Berkes, A., Gore, G., Leimkohl, J.P., Schikora, H., Kollewe, M., Rohde, H., Wilmanns, M., Tellkamp, F., Schulz, E.C.
Millisecond cryo-trapping by the spitrobot crystal plunger simplifies time-resolved crystallography.,Mehrabi P, Sung S, von Stetten D, Prester A, Hatton CE, Kleine-Dopke S, Berkes A, Gore G, Leimkohl JP, Schikora H, Kollewe M, Rohde H, Wilmanns M, Tellkamp F, Schulz EC Nat Commun. 2023 Apr 25;14(1):2365. doi: 10.1038/s41467-023-37834-w. PMID:37185266<ref>PMID:37185266</ref>


Description: Millisecond cryo-trapping by the spitrobot crystal plunger, CTX-M-14 E166A, Ampicillin, 500 MS
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Mehrabi, P]]
<div class="pdbe-citations 8b2w" style="background-color:#fffaf0;"></div>
[[Category: Gore, G]]
== References ==
[[Category: Sung, S]]
<references/>
[[Category: Rohde, H]]
__TOC__
[[Category: Kleine-Doepke, S]]
</StructureSection>
[[Category: Hatton, C.E]]
[[Category: Klebsiella pneumoniae]]
[[Category: Leimkohl, J.P]]
[[Category: Large Structures]]
[[Category: Wilmanns, M]]
[[Category: Berkes A]]
[[Category: Prester, A]]
[[Category: Gore G]]
[[Category: Von Stetten, D]]
[[Category: Hatton CE]]
[[Category: Tellkamp, F]]
[[Category: Kleine-Doepke S]]
[[Category: Kollewe, M]]
[[Category: Kollewe M]]
[[Category: Schikora, H]]
[[Category: Leimkohl JP]]
[[Category: Berkes, A]]
[[Category: Mehrabi P]]
[[Category: Schulz, E.C]]
[[Category: Prester A]]
[[Category: Rohde H]]
[[Category: Schikora H]]
[[Category: Schulz EC]]
[[Category: Sung S]]
[[Category: Tellkamp F]]
[[Category: Wilmanns M]]
[[Category: Von Stetten D]]
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