5a44: Difference between revisions

Latest revision as of 04:38, 25 May 2023

Structure of Bacteriorhodopsin obtained from 20um crystals by multi crystal data collection

Structural highlights

5a44 is a 1 chain structure with sequence from Halobacterium salinarum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	HP6, LI1, RET
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

BACR_HALSA Light-driven proton pump.

Publication Abstract from PubMed

Here, an automated procedure is described to identify the positions of many cryocooled crystals mounted on the same sample holder, to rapidly predict and rank their relative diffraction strengths and to collect partial X-ray diffraction data sets from as many of the crystals as desired. Subsequent hierarchical cluster analysis then allows the best combination of partial data sets, optimizing the quality of the final data set obtained. The results of applying the method developed to various systems and scenarios including the compilation of a complete data set from tiny crystals of the membrane protein bacteriorhodopsin and the collection of data sets for successful structure determination using the single-wavelength anomalous dispersion technique are also presented.

MeshAndCollect: an automated multi-crystal data-collection workflow for synchrotron macromolecular crystallography beamlines.,Zander U, Bourenkov G, Popov AN, de Sanctis D, Svensson O, McCarthy AA, Round E, Gordeliy V, Mueller-Dieckmann C, Leonard GA Acta Crystallogr D Biol Crystallogr. 2015 Nov 1;71(Pt 11):2328-43. doi:, 10.1107/S1399004715017927. Epub 2015 Oct 31. PMID:26527148^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Zander U, Bourenkov G, Popov AN, de Sanctis D, Svensson O, McCarthy AA, Round E, Gordeliy V, Mueller-Dieckmann C, Leonard GA. MeshAndCollect: an automated multi-crystal data-collection workflow for synchrotron macromolecular crystallography beamlines. Acta Crystallogr D Biol Crystallogr. 2015 Nov 1;71(Pt 11):2328-43. doi:, 10.1107/S1399004715017927. Epub 2015 Oct 31. PMID:26527148 doi:https://dx.doi.org/10.1107/S1399004715017927

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Zander U, Bourenkov G, Popov AN, de Sanctis D, Svensson O, McCarthy AA, Round E, Gordeliy V, Mueller-Dieckmann C, Leonard GA. MeshAndCollect: an automated multi-crystal data-collection workflow for synchrotron macromolecular crystallography beamlines. Acta Crystallogr D Biol Crystallogr. 2015 Nov 1;71(Pt 11):2328-43. doi:, 10.1107/S1399004715017927. Epub 2015 Oct 31. PMID:26527148 doi:https://dx.doi.org/10.1107/S1399004715017927

[1]

@@ Line 3: / Line 3: @@
 <StructureSection load='5a44' size='340' side='right'caption='[[5a44]], [[Resolution|resolution]] 2.29&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5a44]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Halobacterium_salinarum Halobacterium salinarum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A44 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5A44 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5a44]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Halobacterium_salinarum Halobacterium salinarum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A44 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5A44 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HP6:HEPTANE'>HP6</scene>, <scene name='pdbligand=LI1:1-[2,6,10.14-TETRAMETHYL-HEXADECAN-16-YL]-2-[2,10,14-TRIMETHYLHEXADECAN-16-YL]GLYCEROL'>LI1</scene>, <scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HP6:HEPTANE'>HP6</scene>, <scene name='pdbligand=LI1:1-[2,6,10.14-TETRAMETHYL-HEXADECAN-16-YL]-2-[2,10,14-TRIMETHYLHEXADECAN-16-YL]GLYCEROL'>LI1</scene>, <scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5a3y|5a3y]], [[5a3z|5a3z]], [[5a45|5a45]], [[5a47|5a47]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5a44 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a44 OCA], [https://pdbe.org/5a44 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5a44 RCSB], [https://www.ebi.ac.uk/pdbsum/5a44 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5a44 ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5a44 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a44 OCA], [http://pdbe.org/5a44 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5a44 RCSB], [http://www.ebi.ac.uk/pdbsum/5a44 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5a44 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/BACR_HALSA BACR_HALSA]] Light-driven proton pump.
+[https://www.uniprot.org/uniprot/BACR_HALSA BACR_HALSA] Light-driven proton pump.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 28: / Line 27: @@
 [[Category: Halobacterium salinarum]]
 [[Category: Large Structures]]
-[[Category: Bourenkov, G]]
+[[Category: Bourenkov G]]
-[[Category: Gordeliy, V I]]
+[[Category: Gordeliy VI]]
-[[Category: Leonard, G A]]
+[[Category: Leonard GA]]
-[[Category: McCarthy, A A]]
+[[Category: McCarthy AA]]
-[[Category: Mueller-Dieckmann, C]]
+[[Category: Mueller-Dieckmann C]]
-[[Category: Popov, A N]]
+[[Category: Popov AN]]
-[[Category: Round, E S]]
+[[Category: Round ES]]
-[[Category: Sanctis, D de]]
+[[Category: Svensson O]]
-[[Category: Svensson, O]]
+[[Category: Zander U]]
-[[Category: Zander, U]]
+[[Category: De Sanctis D]]
-[[Category: 7-helix transmembrane]]
-[[Category: Ion pump]]
-[[Category: Ion transport]]
-[[Category: Lcp]]
-[[Category: Membrane]]
-[[Category: Membrane protein]]
-[[Category: Multi crystal data collection]]
-[[Category: Photoreceptor]]
-[[Category: Proton transport]]
-[[Category: Retinal protein]]
-[[Category: Ssx]]
-[[Category: Synchrotron serial crystallography]]

5a44: Difference between revisions

Latest revision as of 04:38, 25 May 2023

Structure of Bacteriorhodopsin obtained from 20um crystals by multi crystal data collection

Structural highlights

Function

Publication Abstract from PubMed

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References

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