5aor: Difference between revisions

Revision as of 06:20, 31 May 2023

Structure of MLE RNA ADP AlF4 complex

Structural highlights

5aor is a 4 chain structure with sequence from Drosophila melanogaster and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	ADP, ALF, GOL, MG
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

MLE_DROME Required in males for dosage compensation of X chromosome linked genes. Mle, msl-1 and msl-3 are colocalized on X chromosome. Each of the msl proteins requires all the other msls for wild-type X-chromosome binding. Probably unwinds double-stranded DNA and RNA in a 3' to 5' direction.^[1]

Publication Abstract from PubMed

The MLE helicase remodels the roX lncRNAs, enabling the lncRNA-mediated assembly of the Drosophila dosage compensation complex. We identified a stable MLE core comprising the DExH helicase module and two auxiliary domains: a dsRBD and an OB-like fold. MLEcore is an unusual DExH helicase that can unwind blunt-ended RNA duplexes and has specificity for uridine nucleotides. We determined the 2.1 A resolution structure of MLEcore bound to a U10 RNA and ADP-AlF4. The OB-like and dsRBD folds bind the DExH module and contribute to form the entrance of the helicase channel. Four uridine nucleotides engage in base-specific interactions, rationalizing the conservation of uridine-rich sequences in critical roX substrates. roX2 binding is orchestrated by MLE's auxiliary domains, which is prerequisite for MLE localization to the male X chromosome. The structure visualizes a transition-state mimic of the reaction and suggests how eukaryotic DEAH/RHA helicases couple ATP hydrolysis to RNA translocation.

Structure of the RNA Helicase MLE Reveals the Molecular Mechanisms for Uridine Specificity and RNA-ATP Coupling.,Prabu JR, Muller M, Thomae AW, Schussler S, Bonneau F, Becker PB, Conti E Mol Cell. 2015 Nov 5;60(3):487-99. doi: 10.1016/j.molcel.2015.10.011. PMID:26545078^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kuroda MI, Kernan MJ, Kreber R, Ganetzky B, Baker BS. The maleless protein associates with the X chromosome to regulate dosage compensation in Drosophila. Cell. 1991 Sep 6;66(5):935-47. PMID:1653648
↑ Prabu JR, Muller M, Thomae AW, Schussler S, Bonneau F, Becker PB, Conti E. Structure of the RNA Helicase MLE Reveals the Molecular Mechanisms for Uridine Specificity and RNA-ATP Coupling. Mol Cell. 2015 Nov 5;60(3):487-99. doi: 10.1016/j.molcel.2015.10.011. PMID:26545078 doi:https://dx.doi.org/10.1016/j.molcel.2015.10.011

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OCA

[1] Kuroda MI, Kernan MJ, Kreber R, Ganetzky B, Baker BS. The maleless protein associates with the X chromosome to regulate dosage compensation in Drosophila. Cell. 1991 Sep 6;66(5):935-47. PMID:1653648

[2] Prabu JR, Muller M, Thomae AW, Schussler S, Bonneau F, Becker PB, Conti E. Structure of the RNA Helicase MLE Reveals the Molecular Mechanisms for Uridine Specificity and RNA-ATP Coupling. Mol Cell. 2015 Nov 5;60(3):487-99. doi: 10.1016/j.molcel.2015.10.011. PMID:26545078 doi:https://dx.doi.org/10.1016/j.molcel.2015.10.011

[1]

[2]

@@ Line 3: / Line 3: @@
 <StructureSection load='5aor' size='340' side='right'caption='[[5aor]], [[Resolution|resolution]] 2.08&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5aor]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome] and [http://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AOR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AOR FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5aor]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AOR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AOR FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ALF:TETRAFLUOROALUMINATE+ION'>ALF</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ALF:TETRAFLUOROALUMINATE+ION'>ALF</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA_helicase RNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.13 3.6.4.13] </span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5aor FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5aor OCA], [https://pdbe.org/5aor PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5aor RCSB], [https://www.ebi.ac.uk/pdbsum/5aor PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5aor ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5aor FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5aor OCA], [http://pdbe.org/5aor PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5aor RCSB], [http://www.ebi.ac.uk/pdbsum/5aor PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5aor ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/MLE_DROME MLE_DROME]] Required in males for dosage compensation of X chromosome linked genes. Mle, msl-1 and msl-3 are colocalized on X chromosome. Each of the msl proteins requires all the other msls for wild-type X-chromosome binding. Probably unwinds double-stranded DNA and RNA in a 3' to 5' direction.<ref>PMID:1653648</ref>
+[https://www.uniprot.org/uniprot/MLE_DROME MLE_DROME] Required in males for dosage compensation of X chromosome linked genes. Mle, msl-1 and msl-3 are colocalized on X chromosome. Each of the msl proteins requires all the other msls for wild-type X-chromosome binding. Probably unwinds double-stranded DNA and RNA in a 3' to 5' direction.<ref>PMID:1653648</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Drome]]
+[[Category: Drosophila melanogaster]]
 [[Category: Large Structures]]
-[[Category: RNA helicase]]
 [[Category: Synthetic construct]]
-[[Category: Conti, E]]
+[[Category: Conti E]]
-[[Category: Prabu, J R]]
+[[Category: Prabu JR]]
-[[Category: Deah]]
-[[Category: Dosage compensation]]
-[[Category: Helicase]]
-[[Category: Hydrolase-rna complex]]
-[[Category: Mle]]
-[[Category: Rox]]

5aor: Difference between revisions

Revision as of 06:20, 31 May 2023

Structure of MLE RNA ADP AlF4 complex

Structural highlights

Function

Publication Abstract from PubMed

References

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