5c2z

Molecular insights into the specificity of exfoliative toxins from Staphylococcus aureus

spinqualitylabelsall models 5c2z, resolution 1.96Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5c2z is a 2 chain structure with sequence from Staphylococcus aureus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function L0RUV7_STAAU Publication Abstract from PubMed Exfoliative toxins are serine proteases secreted by Staphylococcus aureus that are associated with toxin-mediated staphylococcal syndromes. To date, four different serotypes of exfoliative toxins have been identified and 3 of them (ETA, ETB, and ETD) are linked to human infection. Among these toxins, only the ETD structure remained unknown, limiting our understanding of the structural determinants for the functional differentiation between these toxins. We recently identified an ETD-like protein associated to S. aureus strains involved in mild mastitis in sheep. The crystal structure of this ETD-like protein was determined at 1.95 A resolution and the structural analysis provide insights into the oligomerization, stability and specificity and enabled a comprehensive structural comparison with ETA and ETB. Despite the highly conserved molecular architecture, significant differences in the composition of the loops and in both the N- and C-terminal alpha-helices seem to define ETD-like specificity. Molecular dynamics simulations indicate that these regions defining ET specificity present different degrees of flexibility and may undergo conformational changes upon substrate recognition and binding. DLS and AUC experiments indicated that the ETD-like is monomeric in solution whereas it is present as a dimer in the asymmetric unit indicating that oligomerization is not related to functional differentiation among these toxins. Differential scanning calorimetry and circular dichroism assays demonstrated an endothermic transition centered at 52 degrees C, and an exothermic aggregation in temperatures up to 64 degrees C. All these together provide insights about the mode of action of a toxin often secreted in syndromes that are not associated with either ETA or ETB. Crystal structure of Staphylococcus aureus exfoliative toxin D-like protein: Structural basis for the high specificity of exfoliative toxins.,Mariutti RB, Souza TA, Ullah A, Caruso IP, de Moraes FR, Zanphorlin LM, Tartaglia NR, Seyffert N, Azevedo VA, Le Loir Y, Murakami MT, Arni RK Biochem Biophys Res Commun. 2015 Nov 6;467(1):171-7. doi:, 10.1016/j.bbrc.2015.08.083. Epub 2015 Aug 20. PMID:26299923 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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