5c6f

Crystal structures of ferritin mutants reveal side-on binding to diiron and end-on cleavage of oxygen

spinqualitylabelsall models 5c6f, resolution 2.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5c6f is a 12 chain structure with sequence from Helicobacter pylori J99. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: FE, IMD Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function FTN_HELPJ Iron-storage protein (By similarity). Publication Abstract from PubMed Iron and oxygen chemistry is mediated by iron proteins for many biological functions. Carboxylate-bridged diiron enzymes including ferritin have the common mechanism of oxygen activation via peroxodiferric intermediates. However, the route for iron uptake and the structural identification of intermediates still remain incomplete. The 4-fold symmetry channel of Helicobacter pylori ferritin was previously proposed as the iron-uptake route in eubacteria, but the amino acid residues at the 4-fold channel are not highly conserved. Here, we show evidence for a short path for iron uptake from His93 on the surface to the ferroxidase center in H. pylori ferritin and Escherichia coli ferritin. The amino acid residues along this path are highly conserved in Gram-negative bacteria and some archaea, and the mutants containing S20A and H93L showed significantly decreased iron oxidation. Surprisingly, the E. coli ferritin S20A crystal structure showed oxygen binding and side-on, symmetric mu-eta2:eta2 peroxodiferric and oxodiferric intermediates. The results provide the structural basis for understanding the chemical nature of intermediates in iron oxidation in bacteria and some of archaea. Structural Basis of Novel Iron-Uptake Route and Reaction Intermediates in Ferritins from Gram-Negative Bacteria.,Kim S, Lee JH, Seok JH, Park YH, Jung SW, Cho AE, Lee C, Chung MS, Kim KH J Mol Biol. 2016 Dec 4;428(24 Pt B):5007-5018. doi: 10.1016/j.jmb.2016.10.022., Epub 2016 Oct 21. PMID:27777002 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Ferritin 3D structures

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