2jx8: Difference between revisions

Revision as of 08:38, 14 June 2023

Solution structure of hPCIF1 WW domain

Structural highlights

2jx8 is a 1 chain structure with sequence from Homo sapiens. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CAPAM_HUMAN Cap-specific adenosine methyltransferase that catalyzes formation of N(6),2'-O-dimethyladenosine cap (m6A(m)) by methylating the adenosine at the second transcribed position of capped mRNAs (PubMed:30467178, PubMed:30487554, PubMed:31279658, PubMed:31279659, PubMed:33428944). Recruited to the early elongation complex of RNA polymerase II (RNAPII) via interaction with POLR2A and mediates formation of m6A(m) co-transcriptionally (PubMed:30467178).^[1] ^[2] ^[3] ^[4] ^[5]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

↑ Akichika S, Hirano S, Shichino Y, Suzuki T, Nishimasu H, Ishitani R, Sugita A, Hirose Y, Iwasaki S, Nureki O, Suzuki T. Cap-specific terminal N (6)-methylation of RNA by an RNA polymerase II-associated methyltransferase. Science. 2018 Nov 22. pii: science.aav0080. doi: 10.1126/science.aav0080. PMID:30467178 doi:https://dx.doi.org/10.1126/science.aav0080
↑ Sun H, Zhang M, Li K, Bai D, Yi C. Cap-specific, terminal N(6)-methylation by a mammalian m(6)Am methyltransferase. Cell Res. 2019 Jan;29(1):80-82. PMID:30487554 doi:10.1038/s41422-018-0117-4
↑ Boulias K, Toczydłowska-Socha D, Hawley BR, Liberman N, Takashima K, Zaccara S, Guez T, Vasseur JJ, Debart F, Aravind L, Jaffrey SR, Greer EL. Identification of the m(6)Am Methyltransferase PCIF1 Reveals the Location and Functions of m(6)Am in the Transcriptome. Mol Cell. 2019 Aug 8;75(3):631-643.e8. PMID:31279658 doi:10.1016/j.molcel.2019.06.006
↑ Sendinc E, Valle-Garcia D, Dhall A, Chen H, Henriques T, Navarrete-Perea J, Sheng W, Gygi SP, Adelman K, Shi Y. PCIF1 Catalyzes m6Am mRNA Methylation to Regulate Gene Expression. Mol Cell. 2019 Aug 8;75(3):620-630.e9. PMID:31279659 doi:10.1016/j.molcel.2019.05.030
↑ Yu D, Kaur G, Blumenthal RM, Zhang X, Cheng X. Enzymatic characterization of three human RNA adenosine methyltransferases reveals diverse substrate affinities and reaction optima. J Biol Chem. 2021 Jan-Jun;296:100270. PMID:33428944 doi:10.1016/j.jbc.2021.100270

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OCA

[1] Akichika S, Hirano S, Shichino Y, Suzuki T, Nishimasu H, Ishitani R, Sugita A, Hirose Y, Iwasaki S, Nureki O, Suzuki T. Cap-specific terminal N (6)-methylation of RNA by an RNA polymerase II-associated methyltransferase. Science. 2018 Nov 22. pii: science.aav0080. doi: 10.1126/science.aav0080. PMID:30467178 doi:https://dx.doi.org/10.1126/science.aav0080

[2] Sun H, Zhang M, Li K, Bai D, Yi C. Cap-specific, terminal N(6)-methylation by a mammalian m(6)Am methyltransferase. Cell Res. 2019 Jan;29(1):80-82. PMID:30487554 doi:10.1038/s41422-018-0117-4

[3] Boulias K, Toczydłowska-Socha D, Hawley BR, Liberman N, Takashima K, Zaccara S, Guez T, Vasseur JJ, Debart F, Aravind L, Jaffrey SR, Greer EL. Identification of the m(6)Am Methyltransferase PCIF1 Reveals the Location and Functions of m(6)Am in the Transcriptome. Mol Cell. 2019 Aug 8;75(3):631-643.e8. PMID:31279658 doi:10.1016/j.molcel.2019.06.006

[4] Sendinc E, Valle-Garcia D, Dhall A, Chen H, Henriques T, Navarrete-Perea J, Sheng W, Gygi SP, Adelman K, Shi Y. PCIF1 Catalyzes m6Am mRNA Methylation to Regulate Gene Expression. Mol Cell. 2019 Aug 8;75(3):620-630.e9. PMID:31279659 doi:10.1016/j.molcel.2019.05.030

[5] Yu D, Kaur G, Blumenthal RM, Zhang X, Cheng X. Enzymatic characterization of three human RNA adenosine methyltransferases reveals diverse substrate affinities and reaction optima. J Biol Chem. 2021 Jan-Jun;296:100270. PMID:33428944 doi:10.1016/j.jbc.2021.100270

[1]

[2]

[3]

[4]

[5]

@@ Line 1: / Line 1: @@
 ==Solution structure of hPCIF1 WW domain==
-<StructureSection load='2jx8' size='340' side='right'caption='[[2jx8]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='2jx8' size='340' side='right'caption='[[2jx8]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2jx8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JX8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JX8 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2jx8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JX8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JX8 FirstGlance]. <br>
 </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jx8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jx8 OCA], [https://pdbe.org/2jx8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jx8 RCSB], [https://www.ebi.ac.uk/pdbsum/2jx8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jx8 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/PCIF1_HUMAN PCIF1_HUMAN]] May play a role in transcription elongation or in coupling transcription to pre-mRNA processing through its association with the phosphorylated C-terminal domain (CTD) of RNAPII largest subunit.
+[https://www.uniprot.org/uniprot/CAPAM_HUMAN CAPAM_HUMAN] Cap-specific adenosine methyltransferase that catalyzes formation of N(6),2'-O-dimethyladenosine cap (m6A(m)) by methylating the adenosine at the second transcribed position of capped mRNAs (PubMed:30467178, PubMed:30487554, PubMed:31279658, PubMed:31279659, PubMed:33428944). Recruited to the early elongation complex of RNA polymerase II (RNAPII) via interaction with POLR2A and mediates formation of m6A(m) co-transcriptionally (PubMed:30467178).<ref>PMID:30467178</ref> <ref>PMID:30487554</ref> <ref>PMID:31279658</ref> <ref>PMID:31279659</ref> <ref>PMID:33428944</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 18: / Line 18: @@
 </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2jx8 ConSurf].
 <div style="clear:both"></div>
+== References ==
+<references/>
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
 [[Category: Large Structures]]
-[[Category: Aizawa, T]]
+[[Category: Aizawa T]]
-[[Category: Demura, M]]
+[[Category: Demura M]]
-[[Category: Hirose, Y]]
+[[Category: Hirose Y]]
-[[Category: Iwamoto, Y]]
+[[Category: Iwamoto Y]]
-[[Category: Kawano, K]]
+[[Category: Kawano K]]
-[[Category: Kouno, T]]
+[[Category: Kouno T]]
-[[Category: Mizuguchi, M]]
+[[Category: Mizuguchi M]]
-[[Category: Ohkuma, Y]]
+[[Category: Ohkuma Y]]
-[[Category: Alpha-helix]]
-[[Category: Nucleus]]
-[[Category: Phosphorylation]]
-[[Category: Protein fragment]]
-[[Category: Transcription]]
-[[Category: Triple-standed beta-sheet]]
-[[Category: Ww domain]]

2jx8: Difference between revisions

Revision as of 08:38, 14 June 2023

Solution structure of hPCIF1 WW domain

Structural highlights

Function

Evolutionary Conservation

References

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