1osy

Crystal structure of FIP-Fve fungal immunomodulatory protein

spinqualitylabelsall models 1osy, resolution 1.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 1osy is a 2 chain structure with sequence from Flammulina velutipes. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: ACE, BR Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function FVE_FLAVE Lectin with specificity for complex cell-surface carbohydrates. Possesses immunomodulatory activity, stimulates lymphocyte mitogenesis, suppresses systemic anaphylaxis reactions and edema, enhances transcription of IL-2, IFN-gamma and TNF-alpha and hemagglutinates red blood cells. Publication Abstract from PubMed Fve, a major fruiting body protein from Flammulina velutipes, a mushroom possessing immunomodulatory activity, stimulates lymphocyte mitogenesis, suppresses systemic anaphylaxis reactions and edema, enhances transcription of IL-2, IFN-gamma and TNF-alpha, and hemagglutinates red blood cells. It appears to be a lectin with specificity for complex cell-surface carbohydrates. Fve is a non-covalently linked homodimer containing no Cys, His or Met residues. It shares sequence similarity only to the other fungal immunomodulatory proteins (FIPs) LZ-8, Gts, Vvo and Vvl, all of unknown structure. The 1.7A structure of Fve solved by single anomalous diffraction of NaBr-soaked crystals is novel: each monomer consists of an N-terminal alpha-helix followed by a fibronectin III (FNIII) fold. The FNIII fold is the first instance of "pseudo-h-type" topology, a transition between the seven beta-stranded s-type and the eight beta-stranded h-type topologies. The structure suggests that dimerization, critical for the activity of FIPs, occurs by 3-D domain swapping of the N-terminal helices and is stabilized predominantly by hydrophobic interactions. The structure of Fve is the first in this lectin family to be reported, and the first of an FNIII domain-containing protein of fungal origin. A 1.7A structure of Fve, a member of the new fungal immunomodulatory protein family.,Paaventhan P, Joseph JS, Seow SV, Vaday S, Robinson H, Chua KY, Kolatkar PR J Mol Biol. 2003 Sep 12;332(2):461-70. PMID:12948495 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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