5cd9: Difference between revisions

Revision as of 12:00, 14 June 2023

Crystal structure of the CTD of Drosophila Oskar protein

Structural highlights

5cd9 is a 1 chain structure with sequence from Drosophila melanogaster. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	MSE, SO4
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

OSKA_DROME Organizes the germ plasm and directs localization of the posterior determinant nanos. Oskar protein is required to keep nos RNA and staufen protein at the posterior pole.^[1] ^[2] ^[3]

Publication Abstract from PubMed

Oskar (Osk) protein plays critical roles during Drosophila germ cell development, yet its functions in germ-line formation and body patterning remain poorly understood. This situation contrasts sharply with the vast knowledge about the function and mechanism of osk mRNA localization. Osk is predicted to have an N-terminal LOTUS domain (Osk-N), which has been suggested to bind RNA, and a C-terminal hydrolase-like domain (Osk-C) of unknown function. Here, we report the crystal structures of Osk-N and Osk-C. Osk-N shows a homodimer of winged-helix-fold modules, but without detectable RNA-binding activity. Osk-C has a lipase-fold structure but lacks critical catalytic residues at the putative active site. Surprisingly, we found that Osk-C binds the 3'UTRs of osk and nanos mRNA in vitro. Mutational studies identified a region of Osk-C important for mRNA binding. These results suggest possible functions of Osk in the regulation of stability, regulation of translation, and localization of relevant mRNAs through direct interaction with their 3'UTRs, and provide structural insights into a novel protein-RNA interaction motif involving a hydrolase-related domain.

Structure of Drosophila Oskar reveals a novel RNA binding protein.,Yang N, Yu Z, Hu M, Wang M, Lehmann R, Xu RM Proc Natl Acad Sci U S A. 2015 Sep 15;112(37):11541-6. doi:, 10.1073/pnas.1515568112. Epub 2015 Aug 31. PMID:26324911^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ephrussi A, Lehmann R. Induction of germ cell formation by oskar. Nature. 1992 Jul 30;358(6385):387-92. PMID:1641021 doi:https://dx.doi.org/10.1038/358387a0
↑ Kim-Ha J, Smith JL, Macdonald PM. oskar mRNA is localized to the posterior pole of the Drosophila oocyte. Cell. 1991 Jul 12;66(1):23-35. PMID:2070416
↑ Ephrussi A, Dickinson LK, Lehmann R. Oskar organizes the germ plasm and directs localization of the posterior determinant nanos. Cell. 1991 Jul 12;66(1):37-50. PMID:2070417
↑ Yang N, Yu Z, Hu M, Wang M, Lehmann R, Xu RM. Structure of Drosophila Oskar reveals a novel RNA binding protein. Proc Natl Acad Sci U S A. 2015 Sep 15;112(37):11541-6. doi:, 10.1073/pnas.1515568112. Epub 2015 Aug 31. PMID:26324911 doi:https://dx.doi.org/10.1073/pnas.1515568112

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Ephrussi A, Lehmann R. Induction of germ cell formation by oskar. Nature. 1992 Jul 30;358(6385):387-92. PMID:1641021 doi:https://dx.doi.org/10.1038/358387a0

[2] Kim-Ha J, Smith JL, Macdonald PM. oskar mRNA is localized to the posterior pole of the Drosophila oocyte. Cell. 1991 Jul 12;66(1):23-35. PMID:2070416

[3] Ephrussi A, Dickinson LK, Lehmann R. Oskar organizes the germ plasm and directs localization of the posterior determinant nanos. Cell. 1991 Jul 12;66(1):37-50. PMID:2070417

[4] Yang N, Yu Z, Hu M, Wang M, Lehmann R, Xu RM. Structure of Drosophila Oskar reveals a novel RNA binding protein. Proc Natl Acad Sci U S A. 2015 Sep 15;112(37):11541-6. doi:, 10.1073/pnas.1515568112. Epub 2015 Aug 31. PMID:26324911 doi:https://dx.doi.org/10.1073/pnas.1515568112

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[3]

[4]

@@ Line 3: / Line 3: @@
 <StructureSection load='5cd9' size='340' side='right'caption='[[5cd9]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5cd9]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CD9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5CD9 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5cd9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CD9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CD9 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5cd9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cd9 OCA], [https://pdbe.org/5cd9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5cd9 RCSB], [https://www.ebi.ac.uk/pdbsum/5cd9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5cd9 ProSAT]</span></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5cd7|5cd7]], [[5cd8|5cd8]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5cd9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cd9 OCA], [http://pdbe.org/5cd9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5cd9 RCSB], [http://www.ebi.ac.uk/pdbsum/5cd9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5cd9 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/OSKA_DROME OSKA_DROME]] Organizes the germ plasm and directs localization of the posterior determinant nanos. Oskar protein is required to keep nos RNA and staufen protein at the posterior pole.<ref>PMID:1641021</ref> <ref>PMID:2070416</ref> <ref>PMID:2070417</ref>
+[https://www.uniprot.org/uniprot/OSKA_DROME OSKA_DROME] Organizes the germ plasm and directs localization of the posterior determinant nanos. Oskar protein is required to keep nos RNA and staufen protein at the posterior pole.<ref>PMID:1641021</ref> <ref>PMID:2070416</ref> <ref>PMID:2070417</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 24: / Line 22: @@
 __TOC__
 </StructureSection>
+[[Category: Drosophila melanogaster]]
 [[Category: Large Structures]]
-[[Category: Hu, M]]
+[[Category: Hu M]]
-[[Category: Lehmann, R]]
+[[Category: Lehmann R]]
-[[Category: Wang, M]]
+[[Category: Wang M]]
-[[Category: Xu, R M]]
+[[Category: Xu RM]]
-[[Category: Yang, N]]
+[[Category: Yang N]]
-[[Category: Yu, Z]]
+[[Category: Yu Z]]
-[[Category: 3'-utr]]
-[[Category: Dimerization]]
-[[Category: Rna binding protein]]

5cd9: Difference between revisions

Revision as of 12:00, 14 June 2023

Crystal structure of the CTD of Drosophila Oskar protein

Structural highlights

Function

Publication Abstract from PubMed

References

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