5d50

Crystal structure of Rep-Ant complex from Salmonella-temperate phage

spinqualitylabelsall models 5d50, resolution 2.49Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5d50 is a 16 chain structure with sequence from Salmonella phage SPC32H. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function T1S9Z0_9CAUD Publication Abstract from PubMed DNA-binding repressors are involved in transcriptional repression in many organisms. Disabling a repressor is a crucial step in activating expression of desired genes. Thus, several mechanisms have been identified for the removal of a stably bound repressor (Rep) from the operator. Here, we describe an uncharacterized mechanism of noncanonical DNA binding and induction by a Rep from the temperate Salmonella phage SPC32H; this mechanism was revealed using the crystal structures of homotetrameric Rep (92-198) and a hetero-octameric complex between the Rep and its antirepressor (Ant). The canonical method of inactivating a repressor is through the competitive binding of the antirepressor to the operator-binding site of the repressor; however, these studies revealed several noncanonical features. First, Ant does not compete for the DNA-binding region of Rep. Instead, the tetrameric Ant binds to the C-terminal domains of two asymmetric Rep dimers. Simultaneously, Ant facilitates the binding of the Rep N-terminal domains to Ant, resulting in the release of two Rep dimers from the bound DNA. Second, the dimer pairs of the N-terminal DNA-binding domains originate from different dimers of a Rep tetramer (trans model). This situation is different from that of other canonical Reps, in which two N-terminal DNA-binding domains from the same dimeric unit form a dimer upon DNA binding (cis model). On the basis of these observations, we propose a noncanonical model for the reversible inactivation of a Rep by an Ant. Noncanonical DNA-binding mode of repressor and its disassembly by antirepressor.,Kim M, Kim HJ, Son SH, Yoon HJ, Lim Y, Lee JW, Seok YJ, Jin KS, Yu YG, Kim SK, Ryu S, Lee HH Proc Natl Acad Sci U S A. 2016 May 3;113(18):E2480-8. doi:, 10.1073/pnas.1602618113. Epub 2016 Apr 20. PMID:27099293 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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