5d8w

Structrue of a lucidum protein

spinqualitylabelsall models 5d8w, resolution 2.86Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5d8w is a 2 chain structure with sequence from Ganoderma lucidum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A1A9TAJ1_GANLU Publication Abstract from PubMed Ganoderma lucidum is a saprotrophic white-rot fungus which contains a rich set of cellulolytic enzymes. Here, we screened an array of potential 1,4-beta-endoglucanases from G. lucidum based on the gene annotation library and found that one candidate gene, GlCel5A, exhibits CMC-hydrolyzing activity. The recombinant GlCel5A protein expressed in Pichia pastoris is able to hydrolyze CMC and beta-glucan but not xylan and mannan. The enzyme exhibits optimal activity at 60 degrees C and pH 3-4, and retained 50% activity at 80 and 90 degrees C for at least 15 and 10min. The crystal structure of GlCel5A and its complex with cellobiose, solved at 2.7 and 2.86A resolution, shows a classical (beta/alpha)8 TIM-barrel fold as seen in other members of glycoside hydrolase family 5. The complex structure contains a cellobiose molecule in the +1 and +2 subsites, and reveals the interactions with the positive sites of the enzyme. Collectively, the present work provides the first comprehensive characterization of an endoglucanase from G. lucidum that possesses properties for industrial applications, and strongly encourages further studying in the cellulolytic enzyme system of G. lucidum. Functional and structural analyses of a 1,4-beta-endoglucanase from Ganoderma lucidum.,Liu G, Li Q, Shang N, Huang JW, Ko TP, Liu W, Zheng Y, Han X, Chen Y, Chen CC, Jin J, Guo RT Enzyme Microb Technol. 2016 May;86:67-74. doi: 10.1016/j.enzmictec.2016.01.013., Epub 2016 Feb 1. PMID:26992795 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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