1lc6: Difference between revisions
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'''Solution Structure of the U6 Intramolecular Stem-loop RNA''' | '''Solution Structure of the U6 Intramolecular Stem-loop RNA''' | ||
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==About this Structure== | ==About this Structure== | ||
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LC6 OCA]. | |||
==Reference== | ==Reference== | ||
Metal binding and base ionization in the U6 RNA intramolecular stem-loop structure., Huppler A, Nikstad LJ, Allmann AM, Brow DA, Butcher SE, Nat Struct Biol. 2002 Jun;9(6):431-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11992125 11992125] | Metal binding and base ionization in the U6 RNA intramolecular stem-loop structure., Huppler A, Nikstad LJ, Allmann AM, Brow DA, Butcher SE, Nat Struct Biol. 2002 Jun;9(6):431-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11992125 11992125] | ||
[[Category: Allmann, A M.]] | [[Category: Allmann, A M.]] | ||
[[Category: Brow, D A.]] | [[Category: Brow, D A.]] | ||
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[[Category: Huppler, A.]] | [[Category: Huppler, A.]] | ||
[[Category: Nikstad, L J.]] | [[Category: Nikstad, L J.]] | ||
[[Category: | [[Category: Pentaloop]] | ||
[[Category: | [[Category: Rna]] | ||
[[Category: | [[Category: Stem-loop]] | ||
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 20:46, 2 May 2008
Solution Structure of the U6 Intramolecular Stem-loop RNA
Overview
U6 RNA is a key component of the catalytic core of the spliceosome. A metal ion essential for the first catalytic step of pre-mRNA splicing binds to the U80 Sp phosphate oxygen within the yeast U6 intramolecular stem-loop (ISL). Here we present the first structural data for U6 RNA, revealing the three-dimensional structure of the highly conserved U6 ISL. The ISL binds metal ion at the U80 site with the same stereo specificity as the intact spliceosome. The metal-binding site is adjacent to a readily protonated C.A wobble pair. Protonation of the C.A pair and metal binding are mutually antagonistic. These results support a ribozyme model for U6 RNA function and suggest a possible mechanism for the regulation of RNA splicing.
About this Structure
Full crystallographic information is available from OCA.
Reference
Metal binding and base ionization in the U6 RNA intramolecular stem-loop structure., Huppler A, Nikstad LJ, Allmann AM, Brow DA, Butcher SE, Nat Struct Biol. 2002 Jun;9(6):431-5. PMID:11992125 Page seeded by OCA on Fri May 2 23:46:49 2008
