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==Structure of the Shigella flexneri VapC mutant D98N crystal form 2==
==Structure of the Shigella flexneri VapC mutant D98N crystal form 2==
<StructureSection load='5ecy' size='340' side='right' caption='[[5ecy]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
<StructureSection load='5ecy' size='340' side='right'caption='[[5ecy]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5ecy]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/"shigella_paradysenteriae"_weldin_1927 "shigella paradysenteriae" weldin 1927]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ECY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ECY FirstGlance]. <br>
<table><tr><td colspan='2'>[[5ecy]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ECY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ECY FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5ecd|5ecd]]</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">vapC, mvpA, stborf2, CP0245 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=623 "Shigella paradysenteriae" Weldin 1927])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ecy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ecy OCA], [https://pdbe.org/5ecy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ecy RCSB], [https://www.ebi.ac.uk/pdbsum/5ecy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ecy ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ecy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ecy OCA], [http://pdbe.org/5ecy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ecy RCSB], [http://www.ebi.ac.uk/pdbsum/5ecy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ecy ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/VAPC_SHIFL VAPC_SHIFL]] Toxic component of a toxin-antitoxin (TA) module. A tRNA-(fMet) endonuclease, it cleaves both charged and uncharged tRNA-(fMet) between positions 38 and 39 at the anticodon stem-loop boundary. Does not cleave tRNA(Met), tRNA(Arg2), tRNA(His), tRNA(Leu), tRNA(Phe) tRNA(Thr1), tRNA(Tyr) or tRNA(Val). Overexpression in E.coli inhibits translation, leads to loss of cell growth and degradation of tRNA(fMet); these effects are neutralized by expression of cognate antitoxin VapB. The VapB/VapC complex probably regulates transcription of its own promoter.<ref>PMID:19400780</ref> <ref>PMID:21502523</ref>  Ectopic overexpression in E.coli induces the YoeB toxin, but this is not the cause of VapC toxicity.<ref>PMID:19400780</ref> <ref>PMID:21502523</ref>
[https://www.uniprot.org/uniprot/VAPC_SHIFL VAPC_SHIFL] Toxic component of a toxin-antitoxin (TA) module. A tRNA-(fMet) endonuclease, it cleaves both charged and uncharged tRNA-(fMet) between positions 38 and 39 at the anticodon stem-loop boundary. Does not cleave tRNA(Met), tRNA(Arg2), tRNA(His), tRNA(Leu), tRNA(Phe) tRNA(Thr1), tRNA(Tyr) or tRNA(Val). Overexpression in E.coli inhibits translation, leads to loss of cell growth and degradation of tRNA(fMet); these effects are neutralized by expression of cognate antitoxin VapB. The VapB/VapC complex probably regulates transcription of its own promoter.<ref>PMID:19400780</ref> <ref>PMID:21502523</ref>  Ectopic overexpression in E.coli induces the YoeB toxin, but this is not the cause of VapC toxicity.<ref>PMID:19400780</ref> <ref>PMID:21502523</ref>  
 
==See Also==
*[[Endonuclease 3D structures|Endonuclease 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Shigella paradysenteriae weldin 1927]]
[[Category: Large Structures]]
[[Category: Brodersen, D E]]
[[Category: Shigella flexneri]]
[[Category: Dedic, E]]
[[Category: Brodersen DE]]
[[Category: Xu, K]]
[[Category: Dedic E]]
[[Category: Hydrolase]]
[[Category: Xu K]]
[[Category: Pin-domain]]
[[Category: Toxin]]

Latest revision as of 06:21, 5 July 2023

Structure of the Shigella flexneri VapC mutant D98N crystal form 2

5ecy, resolution 2.00Å

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