8iub

Crystal structure of GH66 endodextranase from Flavobacterium johnsoniae in complex with isomaltotriose

spinqualitylabelsall models 8iub, resolution 1.18Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8iub is a 1 chain structure with sequence from Flavobacterium johnsoniae UW101. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.18Å Ligands: GLC, NA, SO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A5FBI2_FLAJ1 Publication Abstract from PubMed Dextran is an alpha-(1-->6)-glucan that is synthesized by some lactic acid bacteria, and branched dextran with alpha-(1-->2)-, alpha-(1-->3)-, and alpha-(1-->4)-linkages are often produced. Although many dextranases are known to act on the alpha-(1-->6)-linkage of dextran, few studies have functionally analyzed the proteins involved in degrading branched dextran. The mechanism by which bacteria utilize branched dextran is unknown. Earlier, we identified dextranase (FjDex31A) and kojibiose hydrolase (FjGH65A) in the dextran utilization locus (FjDexUL) of a soil Bacteroidota Flavobacterium johnsoniae and hypothesized that FjDexUL is involved in the degradation of alpha-(1-->2)-branched dextran. In this study, we demonstrate that FjDexUL proteins recognize and degrade alpha-(1-->2)- and alpha-(1-->3)-branched dextrans produced by Leuconostoc citreum S-32 (S-32 alpha-glucan). The FjDexUL gene was significantly upregulated when S-32 alpha-glucan was the carbon source compared with alpha-glucooligosaccharides and alpha-glucans, such as linear dextran and branched alpha-glucan from L. citreum S-64. FjDexUL GHs synergistically degraded S-32 alpha-glucan. The crystal structure of FjGH66 shows that some sugar-binding subsites can accommodate alpha-(1-->2)- and alpha-(1-->3)-branches. The structure of FjGH65A in complex with isomaltose supports that FjGH65A acts on alpha-(1-->2)-glucosyl isomaltooligosaccharides. Furthermore, two cell surface sugar-binding proteins (FjDusD and FjDusE) were characterized, and FjDusD showed affinity for isomaltooligosaccharides and FjDusE for dextran, including linear and branched dextrans. Collectively, FjDexUL proteins are suggested to be involved in the degradation of alpha-(1-->2)- and alpha-(1-->3)-branched dextrans. Our results will be helpful in understanding the bacterial nutrient requirements and symbiotic relationships between bacteria at the molecular level. Bacteroidota polysaccharide utilization system for branched dextran exopolysaccharides from lactic acid bacteria.,Nakamura S, Kurata R, Tonozuka T, Funane K, Park EY, Miyazaki T J Biol Chem. 2023 Jun 1:104885. doi: 10.1016/j.jbc.2023.104885. PMID:37269952 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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