5fia

Structure of the effector protein LpiR1 (Lpg0634) from Legionella pneumophila

spinqualitylabelsall models 5fia, resolution 1.75Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5fia is a 2 chain structure with sequence from Legionella pneumophila subsp. pneumophila str. Philadelphia 1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.75Å Ligands: MES, MPD Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q5ZXU7_LEGPH Publication Abstract from PubMed Legionella pneumophila is a causative agent of a severe pneumonia, known as Legionnaires' disease. Legionella's pathogenicity is mediated by specific virulence factors, called bacterial effectors, which are injected into the invaded host cell by the bacterial Type IV Secretion System. Bacterial effectors are involved in complex interactions with the components of the host cell immune and signaling pathways, that eventually lead to bacterial survival and replication inside the mammalian cell. Structural and functional studies of bacterial effectors are therefore crucial for elucidating the mechanisms of Legionella virulence. Here we describe the crystal structure of the LpiR1 (Lpg0634) effector protein and investigate the effects of its overexpression in mammalian cells. LpiR1 is an alpha-helical protein, that consists of two similar domains aligned in an antiparallel fashion. The hydrophilic cleft between the domains might serve as a binding site for a potential host cell interaction partner. LpiR1 binds phosphate group at a conserved site and is stabilized by Mn2+, Ca2+ or Mg2+ ions. When overexpressed in mammalian cells, a GFP-LpiR1 fusion protein is localized in the cytoplasm. Intracellular signaling antibody array analysis revealed small changes in the phosphorylation state of several components of the Akt signaling pathway in HEK293T cells overexpressing LpiR1. Structural and functional investigations of the effector protein LpiR1 from Legionella pneumophila.,Beyrakhova KA, van Straaten K, Li L, Boniecki MT, Anderson DH, Cygler M J Biol Chem. 2016 May 17. pii: jbc.M115.708701. PMID:27226543 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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