8i3j: Difference between revisions

Line 1:

~~'''Unreleased structure'''~~

The entry ~~8i3j~~ is ~~ON HOLD until Paper~~ Publication

==Crystal structure of human inner-arm dynein heavy chain d stalk and microtubule binding domain==

== Structural highlights ==

<table><tr><td colspan='2'>[[8i3j]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8I3J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8I3J FirstGlance]. <br>

</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.69Å</td></tr>

<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8i3j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8i3j OCA], [https://pdbe.org/8i3j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8i3j RCSB], [https://www.ebi.ac.uk/pdbsum/8i3j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8i3j ProSAT]</span></td></tr>

</table>

== Disease ==

[https://www.uniprot.org/uniprot/DYH1_HUMAN DYH1_HUMAN] Non-syndromic male infertility due to sperm motility disorder;Primary ciliary dyskinesia. The disease is caused by variants affecting the gene represented in this entry. The disease is caused by variants affecting the gene represented in this entry.

== Function ==

[https://www.uniprot.org/uniprot/DYH1_HUMAN DYH1_HUMAN] Force generating protein of cilia required for sperm flagellum motility. Produces force towards the minus ends of microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Required in spermatozoa for the formation of the inner dynein arms and biogenesis of the axoneme (PubMed:24360805).[UniProtKB:Q91XQ0]<ref>PMID:24360805</ref>

== Publication Abstract from PubMed ==

Axonemal dynein is an ATP-dependent microtubular motor protein responsible for cilia and flagella beating, and its dysfunction can cause diseases such as primary ciliary dyskinesia and sperm dysmotility. Despite its biological importance, structure-based mechanisms underlying axonemal dynein motors remain unclear. Here, we determined the X-ray crystal structure of the human inner-arm dynein-d (DNAH1) stalk region, which contains a long antiparallel coiled-coil and a microtubule-binding domain (MTBD), at 2.7 A resolution. Notably, differences in the relative orientation of the coiled-coil and MTBD in comparison with other dyneins, as well as the diverse orientations of the MTBD flap region among various isoforms, lead us to propose a 'spike shoe model' with an altered stepping angle for the interaction between IAD-d and microtubules. Based on these findings, we discuss isoform-specific functions of the axonemal dynein stalk MTBDs.

~~Authors~~:

Crystal structure of the stalk region of axonemal inner-arm dynein-d reveals unique features in the coiled-coil and microtubule-binding domain.,Ko S, Toda A, Tanaka H, Yu J, Kurisu G FEBS Lett. 2023 Jul 3. doi: 10.1002/1873-3468.14690. PMID:37400274<ref>PMID:37400274</ref>

~~Description~~:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

[[Category: ~~Unreleased~~ Structures]]

</div>

== References ==

__TOC__

</StructureSection>

[[Category: Homo sapiens]]

[[Category: Large Structures]]

[[Category: Ko S]]

[[Category: Kurisu G]]

[[Category: Tanaka H]]

[[Category: Toda A]]

[[Category: Yu J]]

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 8i3j is ON HOLD  until Paper Publication
+==Crystal structure of human inner-arm dynein heavy chain d stalk and microtubule binding domain==
+<StructureSection load='8i3j' size='340' side='right'caption='[[8i3j]], [[Resolution|resolution]] 2.69&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[8i3j]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8I3J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8I3J FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.69&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8i3j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8i3j OCA], [https://pdbe.org/8i3j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8i3j RCSB], [https://www.ebi.ac.uk/pdbsum/8i3j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8i3j ProSAT]</span></td></tr>
+</table>
+== Disease ==
+[https://www.uniprot.org/uniprot/DYH1_HUMAN DYH1_HUMAN] Non-syndromic male infertility due to sperm motility disorder;Primary ciliary dyskinesia. The disease is caused by variants affecting the gene represented in this entry.  The disease is caused by variants affecting the gene represented in this entry.
+== Function ==
+[https://www.uniprot.org/uniprot/DYH1_HUMAN DYH1_HUMAN] Force generating protein of cilia required for sperm flagellum motility. Produces force towards the minus ends of microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Required in spermatozoa for the formation of the inner dynein arms and biogenesis of the axoneme (PubMed:24360805).[UniProtKB:Q91XQ0]<ref>PMID:24360805</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Axonemal dynein is an ATP-dependent microtubular motor protein responsible for cilia and flagella beating, and its dysfunction can cause diseases such as primary ciliary dyskinesia and sperm dysmotility. Despite its biological importance, structure-based mechanisms underlying axonemal dynein motors remain unclear. Here, we determined the X-ray crystal structure of the human inner-arm dynein-d (DNAH1) stalk region, which contains a long antiparallel coiled-coil and a microtubule-binding domain (MTBD), at 2.7 A resolution. Notably, differences in the relative orientation of the coiled-coil and MTBD in comparison with other dyneins, as well as the diverse orientations of the MTBD flap region among various isoforms, lead us to propose a 'spike shoe model' with an altered stepping angle for the interaction between IAD-d and microtubules. Based on these findings, we discuss isoform-specific functions of the axonemal dynein stalk MTBDs.
-Authors:
+Crystal structure of the stalk region of axonemal inner-arm dynein-d reveals unique features in the coiled-coil and microtubule-binding domain.,Ko S, Toda A, Tanaka H, Yu J, Kurisu G FEBS Lett. 2023 Jul 3. doi: 10.1002/1873-3468.14690. PMID:37400274<ref>PMID:37400274</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 8i3j" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Homo sapiens]]
+[[Category: Large Structures]]
+[[Category: Ko S]]
+[[Category: Kurisu G]]
+[[Category: Tanaka H]]
+[[Category: Toda A]]
+[[Category: Yu J]]