5i68

Alcohol oxidase from Pichia pastoris

Loading... Status: waiting for Mol* initialization Structural highlights 5i68 is a 1 chain structure with sequence from Komagataella pastoris. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.37Å Ligands: FAD, MG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function ALOX1_KOMPG Major isoform of alcohol oxidase, which catalyzes the oxidation of methanol to formaldehyde and hydrogen peroxide, the first step in the methanol utilization pathway of methylotrophic yeasts. Publication Abstract from PubMed The first step in methanol metabolism in methylotrophic yeasts, the oxidation of methanol and higher alcohols with molecular oxygen to formaldehyde and hydrogen peroxide, is catalysed by alcohol oxidase (AOX), a 600-kDa homo-octamer containing eight FAD cofactors. When these yeasts are grown with methanol as the carbon source, AOX forms large crystalline arrays in peroxisomes. We determined the structure of AOX by cryo-electron microscopy at a resolution of 3.4 A. All residues of the 662-amino acid polypeptide as well as the FAD are well resolved. AOX shows high structural homology to other members of the GMC family of oxidoreductases, which share a conserved FAD binding domain, but have different substrate specificities. The preference of AOX for small alcohols is explained by the presence of conserved bulky aromatic residues near the active site. Compared to the other GMC enzymes, AOX contains a large number of amino acid inserts, the longest being 75 residues. These segments are found at the periphery of the monomer and make extensive inter-subunit contacts which are responsible for the very stable octamer. A short surface helix forms contacts between two octamers, explaining the tendency of AOX to form crystals in the peroxisomes. Structure of Alcohol Oxidase from Pichia pastoris by Cryo-Electron Microscopy.,Vonck J, Parcej DN, Mills DJ PLoS One. 2016 Jul 26;11(7):e0159476. doi: 10.1371/journal.pone.0159476., eCollection 2016. PMID:27458710 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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