1mf6: Difference between revisions
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'''Transducin gamma subunit, C-terminal domain 60-71, rhodopsin-bound state: Ensemble of 15 models determined by TrNOE spectroscopy''' | '''Transducin gamma subunit, C-terminal domain 60-71, rhodopsin-bound state: Ensemble of 15 models determined by TrNOE spectroscopy''' | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Kisselev, O G.]] | [[Category: Kisselev, O G.]] | ||
[[Category: | [[Category: Bound conformation]] | ||
[[Category: | [[Category: C-terminal domain]] | ||
[[Category: | [[Category: G-protein]] | ||
[[Category: | [[Category: Gamma subunit]] | ||
[[Category: | [[Category: Gpcr]] | ||
[[Category: | [[Category: Rhodopsin]] | ||
[[Category: | [[Category: Transducin]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:57:45 2008'' | |||
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Revision as of 21:57, 2 May 2008
Transducin gamma subunit, C-terminal domain 60-71, rhodopsin-bound state: Ensemble of 15 models determined by TrNOE spectroscopy
Overview
Rhodopsin, a prototypical G protein-coupled receptor, catalyzes the activation of a heterotrimeric G protein, transducin, to initiate a visual signaling cascade in photoreceptor cells. The betagamma subunit complex, especially the C-terminal domain of the transducin gamma subunit, Gtgamma(60-71)farnesyl, plays a pivotal role in allosteric regulation of nucleotide exchange on the transducin alpha subunit by light-activated rhodopsin. We report that this domain is unstructured in the presence of an inactive receptor but forms an amphipathic helix upon rhodopsin activation. A K65E/E66K charge reversal mutant of the gamma subunit has diminished interactions with the receptor and fails to adopt the helical conformation. The identification of this conformational switch provides a mechanism for active GPCR utilization of the betagamma complex in signal transfer to G proteins.
About this Structure
1MF6 is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
Reference
Rhodopsin controls a conformational switch on the transducin gamma subunit., Kisselev OG, Downs MA, Structure. 2003 Apr;11(4):367-73. PMID:12679015 Page seeded by OCA on Sat May 3 00:57:45 2008
