3dmu: Difference between revisions

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<StructureSection load='3dmu' size='340' side='right'caption='[[3dmu]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
<StructureSection load='3dmu' size='340' side='right'caption='[[3dmu]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3dmu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DMU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3DMU FirstGlance]. <br>
<table><tr><td colspan='2'>[[3dmu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DMU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3DMU FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1ey8|1ey8]], [[1stn|1stn]], [[2rbm|2rbm]], [[3bdc|3bdc]], [[3d4d|3d4d]], [[3c1e|3c1e]], [[3c1f|3c1f]], [[3d6c|3d6c]], [[3e5s|3e5s]], [[3eji|3eji]], [[3ero|3ero]], [[3erq|3erq]], [[3evq|3evq]]</div></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">nuc ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Micrococcal_nuclease Micrococcal nuclease], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.31.1 3.1.31.1] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3dmu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dmu OCA], [https://pdbe.org/3dmu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3dmu RCSB], [https://www.ebi.ac.uk/pdbsum/3dmu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3dmu ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3dmu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dmu OCA], [https://pdbe.org/3dmu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3dmu RCSB], [https://www.ebi.ac.uk/pdbsum/3dmu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3dmu ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/NUC_STAAU NUC_STAAU]] Enzyme that catalyzes the hydrolysis of both DNA and RNA at the 5' position of the phosphodiester bond.  
[https://www.uniprot.org/uniprot/NUC_STAAU NUC_STAAU] Enzyme that catalyzes the hydrolysis of both DNA and RNA at the 5' position of the phosphodiester bond.
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Micrococcal nuclease]]
[[Category: Staphylococcus aureus]]
[[Category: Benning, M]]
[[Category: Benning M]]
[[Category: Garcia-Moreno, E B]]
[[Category: Garcia-Moreno EB]]
[[Category: Isom, D]]
[[Category: Isom D]]
[[Category: Khangulov, V S]]
[[Category: Khangulov VS]]
[[Category: Schlessman, J L]]
[[Category: Schlessman JL]]
[[Category: Calcium]]
[[Category: Endonuclease]]
[[Category: Hydrolase]]
[[Category: Hyperstable variant]]
[[Category: Membrane]]
[[Category: Metal-binding]]
[[Category: Nuclease]]
[[Category: Pdtp]]
[[Category: Secreted]]
[[Category: Staphylococcal nuclease]]
[[Category: Zymogen]]

Latest revision as of 12:49, 30 August 2023

Crystal structure of Staphylococcal nuclease variant PHS T62K at cryogenic temperature

3dmu, resolution 1.80Å

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