3r2c: Difference between revisions

Revision as of 13:10, 30 August 2023

Crystal Structure of Antitermination Factors NusB and NusE in complex with BoxA RNA

Structural highlights

3r2c is a 6 chain structure with sequence from Aquifex aeolicus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.902Å
Ligands:	ACT, PEG
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NUSB_AQUAE Involved in the transcription termination process (By similarity).

Publication Abstract from PubMed

Processive transcription antitermination requires the assembly of the complete antitermination complex, which is initiated by the formation of the ternary NusB-NusE-BoxA RNA complex. We have elucidated the crystal structure of this complex, demonstrating that the BoxA RNA is composed of 8 nt that are recognized by the NusB-NusE heterodimer. Functional biologic and biophysical data support the structural observations and establish the relative significance of key protein-protein and protein-RNA interactions. Further crystallographic investigation of a NusB-NusE-dsRNA complex reveals a heretofore unobserved dsRNA binding site contiguous with the BoxA binding site. We propose that the observed dsRNA represents BoxB RNA, as both single-stranded BoxA and double-stranded BoxB components are present in the classical lambda antitermination site. Combining these data with known interactions amongst antitermination factors suggests a specific model for the assembly of the complete antitermination complex.

Structural basis for RNA recognition by NusB and NusE in the initiation of transcription antitermination.,Stagno JR, Altieri AS, Bubunenko M, Tarasov SG, Li J, Court DL, Byrd RA, Ji X Nucleic Acids Res. 2011 Jun 7. PMID:21652641^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Stagno JR, Altieri AS, Bubunenko M, Tarasov SG, Li J, Court DL, Byrd RA, Ji X. Structural basis for RNA recognition by NusB and NusE in the initiation of transcription antitermination. Nucleic Acids Res. 2011 Jun 7. PMID:21652641 doi:10.1093/nar/gkr418

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Stagno JR, Altieri AS, Bubunenko M, Tarasov SG, Li J, Court DL, Byrd RA, Ji X. Structural basis for RNA recognition by NusB and NusE in the initiation of transcription antitermination. Nucleic Acids Res. 2011 Jun 7. PMID:21652641 doi:10.1093/nar/gkr418

[1]

@@ Line 3: / Line 3: @@
 <StructureSection load='3r2c' size='340' side='right'caption='[[3r2c]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3r2c]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/"aquifex_aeolicus"_huber_and_stetter_2001 "aquifex aeolicus" huber and stetter 2001]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R2C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3R2C FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3r2c]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R2C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3R2C FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.902&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3r2d|3r2d]]</div></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">aq_133, nusB ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 "Aquifex aeolicus" Huber and Stetter 2001]), aq_008, NUSE, rpsJ ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 "Aquifex aeolicus" Huber and Stetter 2001])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3r2c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r2c OCA], [https://pdbe.org/3r2c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3r2c RCSB], [https://www.ebi.ac.uk/pdbsum/3r2c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3r2c ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/NUSB_AQUAE NUSB_AQUAE]] Involved in the transcription termination process (By similarity). [[https://www.uniprot.org/uniprot/RS10_AQUAE RS10_AQUAE]] Involved in the binding of tRNA to the ribosomes.[HAMAP-Rule:MF_00508]
+[https://www.uniprot.org/uniprot/NUSB_AQUAE NUSB_AQUAE] Involved in the transcription termination process (By similarity).
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 27: / Line 26: @@
 __TOC__
 </StructureSection>
-[[Category: Aquifex aeolicus huber and stetter 2001]]
+[[Category: Aquifex aeolicus]]
 [[Category: Large Structures]]
-[[Category: Ji, X]]
+[[Category: Ji X]]
-[[Category: Stagno, J R]]
+[[Category: Stagno JR]]
-[[Category: Cross species nusb-nuse-rna interaction]]
-[[Category: Gene regulation]]
-[[Category: Protein-rna interaction]]
-[[Category: Transcription elongation]]
-[[Category: Transcription-rna complex]]

3r2c: Difference between revisions

Revision as of 13:10, 30 August 2023

Crystal Structure of Antitermination Factors NusB and NusE in complex with BoxA RNA

Structural highlights

Function

Publication Abstract from PubMed

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References

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