5iib: Difference between revisions

Latest revision as of 13:53, 30 August 2023

Crystal structure of red abalone egg VERL repeat 3 in complex with sperm lysin at 1.64 A resolution (crystal form II)

Structural highlights

5iib is a 2 chain structure with sequence from Haliotis rufescens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.64Å
Ligands:	EPE, FRU, GLC, NAG, PRD_900003
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ELYS_HALRU Dissolves the egg vitelline layer nonenzymatically during fertilization. It creates a hole of about 3 mu-m in diameter through which the sperm pass.

Publication Abstract from PubMed

Recognition between sperm and the egg surface marks the beginning of life in all sexually reproducing organisms. This fundamental biological event depends on the species-specific interaction between rapidly evolving counterpart molecules on the gametes. We report biochemical, crystallographic, and mutational studies of domain repeats 1-3 of invertebrate egg coat protein VERL and their interaction with cognate sperm protein lysin. VERL repeats fold like the functionally essential N-terminal repeat of mammalian sperm receptor ZP2, whose structure is also described here. Whereas sequence-divergent repeat 1 does not bind lysin, repeat 3 binds it non-species specifically via a high-affinity, largely hydrophobic interface. Due to its intermediate binding affinity, repeat 2 selectively interacts with lysin from the same species. Exposure of a highly positively charged surface of VERL-bound lysin suggests that complex formation both disrupts the organization of egg coat filaments and triggers their electrostatic repulsion, thereby opening a hole for sperm penetration and fusion.

Structural Basis of Egg Coat-Sperm Recognition at Fertilization.,Raj I, Sadat Al Hosseini H, Dioguardi E, Nishimura K, Han L, Villa A, de Sanctis D, Jovine L Cell. 2017 Jun 15;169(7):1315-1326.e17. doi: 10.1016/j.cell.2017.05.033. PMID:28622512^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Raj I, Sadat Al Hosseini H, Dioguardi E, Nishimura K, Han L, Villa A, de Sanctis D, Jovine L. Structural Basis of Egg Coat-Sperm Recognition at Fertilization. Cell. 2017 Jun 15;169(7):1315-1326.e17. doi: 10.1016/j.cell.2017.05.033. PMID:28622512 doi:https://dx.doi.org/10.1016/j.cell.2017.05.033

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OCA

[1] Raj I, Sadat Al Hosseini H, Dioguardi E, Nishimura K, Han L, Villa A, de Sanctis D, Jovine L. Structural Basis of Egg Coat-Sperm Recognition at Fertilization. Cell. 2017 Jun 15;169(7):1315-1326.e17. doi: 10.1016/j.cell.2017.05.033. PMID:28622512 doi:https://dx.doi.org/10.1016/j.cell.2017.05.033

[1]

@@ Line 1: / Line 1: @@
 ==Crystal structure of red abalone egg VERL repeat 3 in complex with sperm lysin at 1.64 A resolution (crystal form II)==
-<StructureSection load='5iib' size='340' side='right' caption='[[5iib]], [[Resolution|resolution]] 1.64&Aring;' scene=''>
+<StructureSection load='5iib' size='340' side='right'caption='[[5iib]], [[Resolution|resolution]] 1.64&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5iib]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/California_red_abalone California red abalone]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IIB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IIB FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5iib]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Haliotis_rufescens Haliotis rufescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IIB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5IIB FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SUC:SUCROSE'>SUC</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.64&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1lis|1lis]], [[2lis|2lis]], [[1lyn|1lyn]], [[2lyn|2lyn]], [[3d4c|3d4c]], [[3d4g|3d4g]], [[3ef7|3ef7]], [[3nk3|3nk3]], [[3nk4|3nk4]], [[5ii7|5ii7]], [[5ii8|5ii8]], [[5ii9|5ii9]], [[5ii5|5ii5]], [[5ii4|5ii4]], [[5iic|5iic]], [[5iia|5iia]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PRD_900003:sucrose'>PRD_900003</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">VERL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6454 California red abalone])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5iib FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5iib OCA], [https://pdbe.org/5iib PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5iib RCSB], [https://www.ebi.ac.uk/pdbsum/5iib PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5iib ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5iib FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5iib OCA], [http://pdbe.org/5iib PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5iib RCSB], [http://www.ebi.ac.uk/pdbsum/5iib PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5iib ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/ELYS_HALRU ELYS_HALRU]] Dissolves the egg vitelline layer nonenzymatically during fertilization. It creates a hole of about 3 mu-m in diameter through which the sperm pass.
+[https://www.uniprot.org/uniprot/ELYS_HALRU ELYS_HALRU] Dissolves the egg vitelline layer nonenzymatically during fertilization. It creates a hole of about 3 mu-m in diameter through which the sperm pass.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 20: / Line 19: @@
 </div>
 <div class="pdbe-citations 5iib" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Lysin 3D structures|Lysin 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: California red abalone]]
+[[Category: Haliotis rufescens]]
-[[Category: Al-Hosseini, H Sadat]]
+[[Category: Large Structures]]
-[[Category: Jovine, L]]
+[[Category: De Sanctis D]]
-[[Category: Nishimura, K]]
+[[Category: Jovine L]]
-[[Category: Raj, I]]
+[[Category: Nishimura K]]
-[[Category: Sanctis, D De]]
+[[Category: Raj I]]
-[[Category: Cell adhesion]]
+[[Category: Sadat Al-Hosseini H]]
-[[Category: Egg coat]]
-[[Category: Egg coat penetration]]
-[[Category: Egg-sperm interaction]]
-[[Category: Fertilization]]
-[[Category: Gamete recognition]]
-[[Category: Sperm acrosome]]
-[[Category: Sperm receptor]]
-[[Category: Vitelline envelope]]
-[[Category: Zp domain]]
-[[Category: Zp-n domain]]

5iib: Difference between revisions

Latest revision as of 13:53, 30 August 2023

Crystal structure of red abalone egg VERL repeat 3 in complex with sperm lysin at 1.64 A resolution (crystal form II)

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

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