5im5

Crystal structure of designed two-component self-assembling icosahedral cage I53-40

spinqualitylabelsall models 5im5, resolution 3.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5im5 is a 30 chain structure with sequence from Methanocaldococcus jannaschii DSM 2661 and Vibrionales bacterium SWAT-3. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.699Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function RISC_METJA Publication Abstract from PubMed Nature provides many examples of self- and co-assembling protein-based molecular machines, including icosahedral protein cages that serve as scaffolds, enzymes, and compartments for essential biochemical reactions and icosahedral virus capsids, which encapsidate and protect viral genomes and mediate entry into host cells. Inspired by these natural materials, we report the computational design and experimental characterization of co-assembling, two-component, 120-subunit icosahedral protein nanostructures with molecular weights (1.8 to 2.8 megadaltons) and dimensions (24 to 40 nanometers in diameter) comparable to those of small viral capsids. Electron microscopy, small-angle x-ray scattering, and x-ray crystallography show that 10 designs spanning three distinct icosahedral architectures form materials closely matching the design models. In vitro assembly of icosahedral complexes from independently purified components occurs rapidly, at rates comparable to those of viral capsids, and enables controlled packaging of molecular cargo through charge complementarity. The ability to design megadalton-scale materials with atomic-level accuracy and controllable assembly opens the door to a new generation of genetically programmable protein-based molecular machines. Accurate design of megadalton-scale two-component icosahedral protein complexes.,Bale JB, Gonen S, Liu Y, Sheffler W, Ellis D, Thomas C, Cascio D, Yeates TO, Gonen T, King NP, Baker D Science. 2016 Jul 22;353(6297):389-94. doi: 10.1126/science.aaf8818. PMID:27463675 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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