5ipo: Difference between revisions

Revision as of 14:05, 30 August 2023

Solution Structure of Hge36: Scorpine-like Peptide from Hadrurus Gertschi

Structural highlights

5ipo is a 1 chain structure with sequence from Hadrurus gertschi. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

KBX3_HOFGE Has antibacterial activity against B.subtilis, but not against S.aureus. Also has hemolytic and cytolytic activities.^[1] Blocks Kv1.1/KCNA1 (IC(50)=185 nM) potassium channels. Shows a weak hemolytic activity.^[2] ^[3]

Publication Abstract from PubMed

Scorpine-like peptides are two domain peptides found in different scorpion venoms displaying various antimicrobial, cytolytic, and potassium channel-blocking activities. The relative contribution of each domain to their different activities remains to be elucidated. Here, we report the recombinant production, solution structure, and antiparasitic activity of Hge36, first identified as a naturally occurring truncated form of a Scorpine-like peptide from the venom of Hoffmannihadrurus gertschi. We also show that removing the first four residues from Hge36 renders a molecule with enhanced potassium channel-blocking and antiparasitic activities. Our results are important to rationalize the structure-function relationships of a pharmacologically versatile molecular scaffold.

Solution structure and antiparasitic activity of scorpine-like peptides from Hoffmannihadrurus gertschi.,Flores-Solis D, Toledano Y, Rodriguez-Lima O, Cano-Sanchez P, Ramirez-Cordero BE, Landa A, Rodriguez de la Vega RC, Del Rio-Portilla F FEBS Lett. 2016 Jun 17. doi: 10.1002/1873-3468.12255. PMID:27314815^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Diego-Garcia E, Abdel-Mottaleb Y, Schwartz EF, de la Vega RC, Tytgat J, Possani LD. Cytolytic and K+ channel blocking activities of beta-KTx and scorpine-like peptides purified from scorpion venoms. Cell Mol Life Sci. 2008 Jan;65(1):187-200. PMID:18030427 doi:https://dx.doi.org/10.1007/s00018-007-7370-x
↑ Diego-Garcia E, Abdel-Mottaleb Y, Schwartz EF, de la Vega RC, Tytgat J, Possani LD. Cytolytic and K+ channel blocking activities of beta-KTx and scorpine-like peptides purified from scorpion venoms. Cell Mol Life Sci. 2008 Jan;65(1):187-200. PMID:18030427 doi:https://dx.doi.org/10.1007/s00018-007-7370-x
↑ Flores-Solis D, Toledano Y, Rodriguez-Lima O, Cano-Sanchez P, Ramirez-Cordero BE, Landa A, Rodriguez de la Vega RC, Del Rio-Portilla F. Solution structure and antiparasitic activity of scorpine-like peptides from Hoffmannihadrurus gertschi. FEBS Lett. 2016 Jun 17. doi: 10.1002/1873-3468.12255. PMID:27314815 doi:https://dx.doi.org/10.1002/1873-3468.12255
↑ Flores-Solis D, Toledano Y, Rodriguez-Lima O, Cano-Sanchez P, Ramirez-Cordero BE, Landa A, Rodriguez de la Vega RC, Del Rio-Portilla F. Solution structure and antiparasitic activity of scorpine-like peptides from Hoffmannihadrurus gertschi. FEBS Lett. 2016 Jun 17. doi: 10.1002/1873-3468.12255. PMID:27314815 doi:https://dx.doi.org/10.1002/1873-3468.12255

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OCA

[1] Diego-Garcia E, Abdel-Mottaleb Y, Schwartz EF, de la Vega RC, Tytgat J, Possani LD. Cytolytic and K+ channel blocking activities of beta-KTx and scorpine-like peptides purified from scorpion venoms. Cell Mol Life Sci. 2008 Jan;65(1):187-200. PMID:18030427 doi:https://dx.doi.org/10.1007/s00018-007-7370-x

[2] Diego-Garcia E, Abdel-Mottaleb Y, Schwartz EF, de la Vega RC, Tytgat J, Possani LD. Cytolytic and K+ channel blocking activities of beta-KTx and scorpine-like peptides purified from scorpion venoms. Cell Mol Life Sci. 2008 Jan;65(1):187-200. PMID:18030427 doi:https://dx.doi.org/10.1007/s00018-007-7370-x

[3] Flores-Solis D, Toledano Y, Rodriguez-Lima O, Cano-Sanchez P, Ramirez-Cordero BE, Landa A, Rodriguez de la Vega RC, Del Rio-Portilla F. Solution structure and antiparasitic activity of scorpine-like peptides from Hoffmannihadrurus gertschi. FEBS Lett. 2016 Jun 17. doi: 10.1002/1873-3468.12255. PMID:27314815 doi:https://dx.doi.org/10.1002/1873-3468.12255

[4] Flores-Solis D, Toledano Y, Rodriguez-Lima O, Cano-Sanchez P, Ramirez-Cordero BE, Landa A, Rodriguez de la Vega RC, Del Rio-Portilla F. Solution structure and antiparasitic activity of scorpine-like peptides from Hoffmannihadrurus gertschi. FEBS Lett. 2016 Jun 17. doi: 10.1002/1873-3468.12255. PMID:27314815 doi:https://dx.doi.org/10.1002/1873-3468.12255

[1]

[2]

[3]

[4]

@@ Line 1: / Line 1: @@
 ==Solution Structure of Hge36: Scorpine-like Peptide from Hadrurus Gertschi==
-<StructureSection load='5ipo' size='340' side='right'caption='[[5ipo]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='5ipo' size='340' side='right'caption='[[5ipo]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5ipo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Hofge Hofge]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IPO OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5IPO FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5ipo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Hadrurus_gertschi Hadrurus gertschi]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IPO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5IPO FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5ipo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ipo OCA], [http://pdbe.org/5ipo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ipo RCSB], [http://www.ebi.ac.uk/pdbsum/5ipo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ipo ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ipo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ipo OCA], [https://pdbe.org/5ipo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ipo RCSB], [https://www.ebi.ac.uk/pdbsum/5ipo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ipo ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/KBX3_HADGE KBX3_HADGE]] Hge-scorpine has antibacterial activity against B.subtilis, but not against S.aureus. Also has hemolytic and cytolytic activities.<ref>PMID:18030427</ref>   Hge36 peptide blocks Kv1.1/KCNA1 (IC(50)=185 nM) potassium channels. Shows a weak hemolytic activity.<ref>PMID:18030427</ref>
+[https://www.uniprot.org/uniprot/KBX3_HOFGE KBX3_HOFGE] Has antibacterial activity against B.subtilis, but not against S.aureus. Also has hemolytic and cytolytic activities.<ref>PMID:18030427</ref>   Blocks Kv1.1/KCNA1 (IC(50)=185 nM) potassium channels. Shows a weak hemolytic activity.<ref>PMID:18030427</ref> <ref>PMID:27314815</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Hofge]]
+[[Category: Hadrurus gertschi]]
 [[Category: Large Structures]]
-[[Category: Flores-Solis, D]]
+[[Category: Flores-Solis D]]
-[[Category: Rio-Portilla, F del]]
+[[Category: Rodriguez De La Vega R]]
-[[Category: Vega, R Rodriguez De La]]
+[[Category: Del Rio-Portilla F]]
-[[Category: Antiparasitic activity]]
-[[Category: Hadrurus gertschi]]
-[[Category: Scorpine-like peptide]]
-[[Category: Toxin]]

5ipo: Difference between revisions

Revision as of 14:05, 30 August 2023

Solution Structure of Hge36: Scorpine-like Peptide from Hadrurus Gertschi

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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