3ku2: Difference between revisions

Latest revision as of 08:23, 6 September 2023

Crystal Structure of inactivated form of CDPK1 from toxoplasma gondii, TGME49.101440

Structural highlights

3ku2 is a 1 chain structure with sequence from Toxoplasma gondii. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.3Å
Ligands:	ANP, UNX
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q9BJF5_TOXGO

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Calcium-dependent protein kinases (CDPKs) have pivotal roles in the calcium-signaling pathway in plants, ciliates and apicomplexan parasites and comprise a calmodulin-dependent kinase (CaMK)-like kinase domain regulated by a calcium-binding domain in the C terminus. To understand this intramolecular mechanism of activation, we solved the structures of the autoinhibited (apo) and activated (calcium-bound) conformations of CDPKs from the apicomplexan parasites Toxoplasma gondii and Cryptosporidium parvum. In the apo form, the C-terminal CDPK activation domain (CAD) resembles a calmodulin protein with an unexpected long helix in the N terminus that inhibits the kinase domain in the same manner as CaMKII. Calcium binding triggers the reorganization of the CAD into a highly intricate fold, leading to its relocation around the base of the kinase domain to a site remote from the substrate binding site. This large conformational change constitutes a distinct mechanism in calcium signal-transduction pathways.

Structures of apicomplexan calcium-dependent protein kinases reveal mechanism of activation by calcium.,Wernimont AK, Artz JD, Finerty P Jr, Lin YH, Amani M, Allali-Hassani A, Senisterra G, Vedadi M, Tempel W, Mackenzie F, Chau I, Lourido S, Sibley LD, Hui R Nat Struct Mol Biol. 2010 May;17(5):596-601. Epub 2010 May 2. PMID:20436473^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Wernimont AK, Artz JD, Finerty P Jr, Lin YH, Amani M, Allali-Hassani A, Senisterra G, Vedadi M, Tempel W, Mackenzie F, Chau I, Lourido S, Sibley LD, Hui R. Structures of apicomplexan calcium-dependent protein kinases reveal mechanism of activation by calcium. Nat Struct Mol Biol. 2010 May;17(5):596-601. Epub 2010 May 2. PMID:20436473 doi:10.1038/nsmb.1795

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Wernimont AK, Artz JD, Finerty P Jr, Lin YH, Amani M, Allali-Hassani A, Senisterra G, Vedadi M, Tempel W, Mackenzie F, Chau I, Lourido S, Sibley LD, Hui R. Structures of apicomplexan calcium-dependent protein kinases reveal mechanism of activation by calcium. Nat Struct Mol Biol. 2010 May;17(5):596-601. Epub 2010 May 2. PMID:20436473 doi:10.1038/nsmb.1795

[1]

@@ Line 3: / Line 3: @@
 <StructureSection load='3ku2' size='340' side='right'caption='[[3ku2]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3ku2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Toxgo Toxgo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KU2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3KU2 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3ku2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Toxoplasma_gondii Toxoplasma gondii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KU2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3KU2 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CDPK1, TGME49_101440 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5811 TOXGO])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ku2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ku2 OCA], [http://pdbe.org/3ku2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ku2 RCSB], [http://www.ebi.ac.uk/pdbsum/3ku2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ku2 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ku2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ku2 OCA], [https://pdbe.org/3ku2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ku2 RCSB], [https://www.ebi.ac.uk/pdbsum/3ku2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ku2 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q9BJF5_TOXGO Q9BJF5_TOXGO]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 35: / Line 37: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Toxgo]]
+[[Category: Toxoplasma gondii]]
-[[Category: Arrowsmith, C H]]
+[[Category: Arrowsmith CH]]
-[[Category: Artz, J D]]
+[[Category: Artz JD]]
-[[Category: Bochkarev, A]]
+[[Category: Bochkarev A]]
-[[Category: Bountra, C]]
+[[Category: Bountra C]]
-[[Category: Edwards, A M]]
+[[Category: Edwards AM]]
-[[Category: Finnerty, P]]
+[[Category: Finnerty P]]
-[[Category: Hassani, A A]]
+[[Category: Hassani AA]]
-[[Category: He, H]]
+[[Category: He H]]
-[[Category: Hui, R]]
+[[Category: Hui R]]
-[[Category: Lin, Y H]]
+[[Category: Lin YH]]
-[[Category: Lourido, S]]
+[[Category: Lourido S]]
-[[Category: Mackenzie, F]]
+[[Category: Mackenzie F]]
-[[Category: Structural genomic]]
+[[Category: Sibley DL]]
-[[Category: Sibley, D L]]
+[[Category: Sinestera G]]
-[[Category: Sinestera, G]]
+[[Category: Vedadi M]]
-[[Category: Vedadi, M]]
+[[Category: Wasney G]]
-[[Category: Wasney, G]]
+[[Category: Weigelt J]]
-[[Category: Weigelt, J]]
+[[Category: Wernimont AK]]
-[[Category: Wernimont, A K]]
+[[Category: Xiao T]]
-[[Category: Xiao, T]]
-[[Category: Atp-binding]]
-[[Category: Cdpk]]
-[[Category: Nucleotide-binding]]
-[[Category: Protist]]
-[[Category: Serine/threonine-protein kinase]]
-[[Category: Sgc]]
-[[Category: Toxoplasma]]
-[[Category: Transferase]]

3ku2: Difference between revisions

Latest revision as of 08:23, 6 September 2023

Crystal Structure of inactivated form of CDPK1 from toxoplasma gondii, TGME49.101440

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

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