3mav

Crystal structure of Plasmodium vivax putative farnesyl pyrophosphate synthase (Pv092040)

spinqualitylabelsall models 3mav, resolution 2.10Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3mav is a 4 chain structure with sequence from Plasmodium vivax Sal-1. This structure supersedes the now removed PDB entry 2ihi. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.1Å Ligands: SO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A5K4U6_PLAVS Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed We present herein a study of a eukaryotic trans-prenylsynthase from the malaria pathogen Plasmodium vivax. Based on the results of biochemical assays and contrary to previous indications, this enzyme catalyzes the production of geranylgeranyl pyrophosphate (GGPP) rather than farnesyl pyrophosphate (FPP). Structural analysis shows that the product length is constrained by a hydrophobic cavity formed primarily by a set of residues from the same subunit as the product as well as at least one other from the dimeric partner. Furthermore, Plasmodium GGPPS can bind nitrogen-containing bisphosphonates (N-BP) strongly with the energetically favorable cooperation of three Mg2+, resulting in inhibition by this class of compounds at IC50 concentrations below 100 nM. In contrast, human and yeast GGPPSs do not accommodate a third magnesium atom in the same manner, resulting in their insusceptibility to N-BPs. This differentiation is in part attributable to a deviation in a conserved motif known as the Second Aspartate-Rich Motif (SARM): whereas the aspartates at the start and end of the five-residue motif in FPPSs and PvGGPPS both participate in the coordination of the third Mg2+, an asparagine is featured as the last residue in human and yeast GGPPSs, resulting in a different manner of interaction with nitrogen-containing ligands. Molecular characterization of a novel geranylgeranyl pyrophosphate synthase from Plasmodium parasites.,Artz JD, Wernimont AK, Dunford JE, Schapira M, Dong A, Zhao Y, Lew J, Russell RG, Ebetino FH, Oppermann U, Hui R J Biol Chem. 2010 Nov 17. PMID:21084289 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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