4kdp

TcaR-ssDNA complex crystal structure reveals the novel ssDNA binding mechanism of the MarR family proteins

spinqualitylabelsall models 4kdp, resolution 3.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4kdp is a 9 chain structure with sequence from Staphylococcus epidermidis ATCC 12228. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.6Å Ligands: EDO, TRS Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A0H2VJZ8_STAES Publication Abstract from PubMed The teicoplanin-associated locus regulator (TcaR) regulates gene expression of proteins on the intercellular adhesion (ica) locus involved in staphylococci poly-N-acetylglucosamine biosynthesis. The absence of TcaR increases poly-N-acetylglucosamine production and promotes biofilm formation. Until recently, the mechanism of multiple antibiotic resistance regulator family protein members, such as TcaR, was restricted to binding double-stranded DNA. However, we recently found that TcaR strongly interacts with single-stranded DNA, which is a new role for this family of proteins. In this study, we report Staphylococcus epidermidis TcaR-single-stranded DNA complex structures. Our model suggests that TcaR and single-stranded DNA form a 61-symmetry polymer composed of TcaR dimers with single-stranded DNA that wraps outside the polymer and 12 nt per TcaR dimer. Single-stranded DNA binding to TcaR involves a large conformational change at the DNA binding lobe. Several point mutations involving the single-stranded DNA binding surface validate interactions between single-stranded DNA and TcaR. Our results extend the novel role of multiple antibiotic resistance regulator family proteins in staphylococci. TcaR-ssDNA complex crystal structure reveals new DNA binding mechanism of the MarR family proteins.,Chang YM, Ho CH, Chen CK, Maestre-Reyna M, Chang-Chien MW, Wang AH Nucleic Acids Res. 2014 Feb 14. PMID:24531929 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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