4liw

CcmK1 Carboxysome Shell Protein from Synechocystis PCC6803, L11K Point Mutant

spinqualitylabelsall models 4liw, resolution 1.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4liw is a 2 chain structure with sequence from Synechocystis sp. PCC 6803 substr. Kazusa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.6Å Ligands: SO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function CCMK1_SYNY3 May be involved in the formation of the carboxysome, a polyhedral inclusion where RuBisCO is sequestered (By similarity). Publication Abstract from PubMed The carboxysome is a giant protein complex that acts as a metabolic organelle in cyanobacteria and some chemoautotrophs. Its outer structure is formed by the assembly of thousands of copies of hexameric shell protein subunits into a molecular layer. The structure determination of a CcmK1 shell protein mutant (L11K) from the beta-carboxysome of the cyanobacterium Synechocystis PCC6803 led to challenges in structure determination. Twinning, noncrystallographic symmetry and packing of hexameric units in a special arrangement led to initial difficulties in space-group assignment. The correct space group was clarified after initial model refinement revealed additional symmetry. This study provides an instructive example in which broken symmetry requires a new choice of unit-cell origin in order to identify the highest symmetry space group. An additional observation related to the packing arrangement of molecules in this crystal suggests that these hexameric shell proteins might have lower internal symmetry than previously believed. A challenging interpretation of a hexagonally layered protein structure.,Thompson MC, Yeates TO Acta Crystallogr D Biol Crystallogr. 2014 Jan;70(Pt 1):203-8. doi:, 10.1107/S139900471302422X. Epub 2013 Dec 24. PMID:24419393 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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