5jrc

Crystal structure of NeC3PO in complex with ssRNA.

spinqualitylabelsall models 5jrc, resolution 1.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5jrc is a 5 chain structure with sequence from Nanoarchaeum equitans Kin4-M and Unidentified. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.9Å Ligands: CA Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q74ML9_NANEQ Publication Abstract from PubMed Translin and translin-associated factor-x are highly conserved in eukaroytes; they can form heteromeric complexes (known as C3POs) and participate in various nucleic acid metabolism pathways. In humans and Drosophila, C3POs cleave the fragmented siRNA passenger strands and facilitate the activation of RNA-induced silencing complex, the effector complex of RNA interference (RNAi). Here, we report three crystal structures of Nanoarchaeum equitans (Ne) C3PO. The apo-NeC3PO structure adopts an open form and unravels a potential substrates entryway for the first time. The NeC3PO:ssRNA and NeC3PO:ssDNA complexes fold like closed football with the substrates captured at the inner cavities. The NeC3PO:ssRNA structure represents the only catalytic form C3PO complex available to date; with mutagenesis and in vitro cleavage assays, the structure provides critical insights into the substrate binding and the two-cation-assisted catalytic mechanisms that are shared by eukaryotic C3POs. The work presented here further advances our understanding on the RNAi pathway. Structural basis for single-stranded RNA recognition and cleavage by C3PO.,Zhang J, Liu H, Yao Q, Yu X, Chen Y, Cui R, Wu B, Zheng L, Zuo J, Huang Z, Ma J, Gan J Nucleic Acids Res. 2016 Sep 4. pii: gkw776. PMID:27596600 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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