6d0k: Difference between revisions

Latest revision as of 15:15, 4 October 2023

Crystal structure of a CLC-type fluoride/proton antiporter, E118Q mutant

Structural highlights

6d0k is a 4 chain structure with sequence from Enterococcus casseliflavus EC10 and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.35Å
Ligands:	DMU, F, MHA
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

C9CPP6_ENTCS

Publication Abstract from PubMed

Fluoride/proton antiporters of the CLC(F) family combat F(-) toxicity in bacteria by exporting this halide from the cytoplasm. These transporters belong to the widespread CLC superfamily but display transport properties different from those of the well-studied Cl(-)/H(+) antiporters. Here, we report a structural and functional investigation of these F(-)-transport proteins. Crystal structures of a CLC(F) homolog from Enterococcus casseliflavus are captured in two conformations with simultaneous accessibility of F(-) and H(+) ions via separate pathways on opposite sides of the membrane. Manipulation of a key glutamate residue critical for H(+) and F(-) transport reverses the anion selectivity of transport; replacement of the glutamate with glutamine or alanine completely inhibits F(-) and H(+) transport while allowing for rapid uncoupled flux of Cl(-). The structural and functional results lead to a 'windmill' model of CLC antiport wherein F (-) and H(+) simultaneously move through separate ion-specific pathways that switch sidedness during the transport cycle.

A CLC-type F(-)/H(+) antiporter in ion-swapped conformations.,Last NB, Stockbridge RB, Wilson AE, Shane T, Kolmakova-Partensky L, Koide A, Koide S, Miller C Nat Struct Mol Biol. 2018 Jun 25. pii: 10.1038/s41594-018-0082-0. doi:, 10.1038/s41594-018-0082-0. PMID:29941917^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Last NB, Stockbridge RB, Wilson AE, Shane T, Kolmakova-Partensky L, Koide A, Koide S, Miller C. A CLC-type F(-)/H(+) antiporter in ion-swapped conformations. Nat Struct Mol Biol. 2018 Jun 25. pii: 10.1038/s41594-018-0082-0. doi:, 10.1038/s41594-018-0082-0. PMID:29941917 doi:https://dx.doi.org/10.1038/s41594-018-0082-0

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Last NB, Stockbridge RB, Wilson AE, Shane T, Kolmakova-Partensky L, Koide A, Koide S, Miller C. A CLC-type F(-)/H(+) antiporter in ion-swapped conformations. Nat Struct Mol Biol. 2018 Jun 25. pii: 10.1038/s41594-018-0082-0. doi:, 10.1038/s41594-018-0082-0. PMID:29941917 doi:https://dx.doi.org/10.1038/s41594-018-0082-0

[1]

@@ Line 3: / Line 3: @@
 <StructureSection load='6d0k' size='340' side='right'caption='[[6d0k]], [[Resolution|resolution]] 3.35&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6d0k]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Entcs Entcs] and [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6D0K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6D0K FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6d0k]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_casseliflavus_EC10 Enterococcus casseliflavus EC10] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6D0K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6D0K FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DMU:DECYL-BETA-D-MALTOPYRANOSIDE'>DMU</scene>, <scene name='pdbligand=F:FLUORIDE+ION'>F</scene>, <scene name='pdbligand=MHA:(CARBAMOYLMETHYL-CARBOXYMETHYL-AMINO)-ACETIC+ACID'>MHA</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.35&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ECAG_02710 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=565654 ENTCS])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DMU:DECYL-BETA-D-MALTOPYRANOSIDE'>DMU</scene>, <scene name='pdbligand=F:FLUORIDE+ION'>F</scene>, <scene name='pdbligand=MHA:(CARBAMOYLMETHYL-CARBOXYMETHYL-AMINO)-ACETIC+ACID'>MHA</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6d0k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6d0k OCA], [http://pdbe.org/6d0k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6d0k RCSB], [http://www.ebi.ac.uk/pdbsum/6d0k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6d0k ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6d0k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6d0k OCA], [https://pdbe.org/6d0k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6d0k RCSB], [https://www.ebi.ac.uk/pdbsum/6d0k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6d0k ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/C9CPP6_ENTCS C9CPP6_ENTCS]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Entcs]]
+[[Category: Enterococcus casseliflavus EC10]]
 [[Category: Large Structures]]
-[[Category: Synthetic construct sequences]]
+[[Category: Synthetic construct]]
-[[Category: Koide, A]]
+[[Category: Koide A]]
-[[Category: Koide, S]]
+[[Category: Koide S]]
-[[Category: Kolmakova-Partensky, L]]
+[[Category: Kolmakova-Partensky L]]
-[[Category: Last, N B]]
+[[Category: Last NB]]
-[[Category: Miller, C]]
+[[Category: Miller C]]
-[[Category: Shane, T]]
+[[Category: Shane T]]
-[[Category: Stockbridge, R B]]
+[[Category: Stockbridge RB]]
-[[Category: Wilson, A E]]
+[[Category: Wilson AE]]
-[[Category: Clc membrane protein]]
-[[Category: Fluoride/proton antiporter]]
-[[Category: Transport protein]]

6d0k: Difference between revisions

Latest revision as of 15:15, 4 October 2023

Crystal structure of a CLC-type fluoride/proton antiporter, E118Q mutant

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools