6n8e

Crystal structure of holo-ObiF1, a five domain nonribosomal peptide synthetase from Burkholderia diffusa

spinqualitylabelsall models 6n8e, resolution 3.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6n8e is a 1 chain structure with sequence from Burkholderia diffusa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3Å Ligands: CL, KFG, KFJ, PNS, PO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A5H1ZR44_9BURK Publication Abstract from PubMed Nonribosomal peptide synthetases produce diverse natural products using a multidomain architecture where the growing peptide, attached to an integrated carrier domain, is delivered to neighboring catalytic domains for bond formation and modification. Investigation of these systems can lead to the discovery of new structures, unusual biosynthetic transformations, and to the engineering of catalysts for generating new products. The antimicrobial beta-lactone obafluorin is produced nonribosomally from dihydroxybenzoic acid and a beta-hydroxy amino acid that cyclizes into the beta-lactone during product release. Here we report the structure of the nonribosomal peptide synthetase ObiF1, highlighting the structure of the beta-lactone-producing thioesterase domain and an interaction between the C-terminal MbtH-like domain with an upstream adenylation domain. Biochemical assays examine catalytic promiscuity, provide mechanistic insight, and demonstrate utility for generating obafluorin analogs. These results advance our understanding of the structural cycle of nonribosomal peptide synthetases and provide insights into the production of beta-lactone natural products. The structural basis of N-acyl-alpha-amino-beta-lactone formation catalyzed by a nonribosomal peptide synthetase.,Kreitler DF, Gemmell EM, Schaffer JE, Wencewicz TA, Gulick AM Nat Commun. 2019 Jul 31;10(1):3432. doi: 10.1038/s41467-019-11383-7. PMID:31366889 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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