6o8l

Crystal Structure of C9S apo and reduced Sulfide-responsive transcriptional repressor (SqrR) from Rhodobacter capsulatus.

spinqualitylabelsall models 6o8l, resolution 1.36Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6o8l is a 1 chain structure with sequence from Rhodobacter capsulatus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.36Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function D5AT91_RHOCB Publication Abstract from PubMed Cysteine thiol-based transcriptional regulators orchestrate the coordinated regulation of redox homeostasis and other cellular processes by 'sensing' or detecting a specific redox-active molecule, which in turn activates the transcription of a specific detoxification pathway. The extent to which these sensors are truly specific in cells for a singular class of reactive small-molecule stressors, for example, reactive oxygen or sulfur species, is largely unknown. Here, we report structural and mechanistic insights into the thiol-based transcriptional repressor SqrR, which reacts exclusively with oxidized sulfur species such as persulfides, to yield a tetrasulfide bridge that inhibits DNA operator-promoter binding. Evaluation of crystallographic structures of SqrR in various derivatized states, coupled with the results of a mass spectrometry-based kinetic profiling strategy, suggest that persulfide selectivity is determined by structural frustration of the disulfide form. These findings led to the identification of an uncharacterized repressor from the bacterial pathogen Acinetobacter baumannii as a persulfide sensor. Structural basis for persulfide-sensing specificity in a transcriptional regulator.,Capdevila DA, Walsh BJC, Zhang Y, Dietrich C, Gonzalez-Gutierrez G, Giedroc DP Nat Chem Biol. 2020 Oct 26. pii: 10.1038/s41589-020-00671-9. doi:, 10.1038/s41589-020-00671-9. PMID:33106663 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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