5lsi: Difference between revisions

Latest revision as of 18:45, 18 October 2023

CRYSTAL STRUCTURE OF THE KINETOCHORE MIS12 COMPLEX HEAD2 SUBDOMAIN CONTAINING DSN1 AND NSL1 FRAGMENTS

Structural highlights

5lsi is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.002Å
Ligands:	SO4
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

DSN1_HUMAN Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis.^[1] ^[2]

Publication Abstract from PubMed

Kinetochores, multisubunit protein assemblies, connect chromosomes to spindle microtubules to promote chromosome segregation. The 10-subunit KMN assembly (comprising KNL1, MIS12, and NDC80 complexes, designated KNL1C, MIS12C, and NDC80C) binds microtubules and regulates mitotic checkpoint function through NDC80C and KNL1C, respectively. MIS12C, on the other hand, connects the KMN to the chromosome-proximal domain of the kinetochore through a direct interaction with CENP-C. The structural basis for this crucial bridging function of MIS12C is unknown. Here, we report crystal structures of human MIS12C associated with a fragment of CENP-C and unveil the role of Aurora B kinase in the regulation of this interaction. The structure of MIS12:CENP-C complements previously determined high-resolution structures of functional regions of NDC80C and KNL1C and allows us to build a near-complete structural model of the KMN assembly. Our work illuminates the structural organization of essential chromosome segregation machinery that is conserved in most eukaryotes.

Structure of the MIS12 Complex and Molecular Basis of Its Interaction with CENP-C at Human Kinetochores.,Petrovic A, Keller J, Liu Y, Overlack K, John J, Dimitrova YN, Jenni S, van Gerwen S, Stege P, Wohlgemuth S, Rombaut P, Herzog F, Harrison SC, Vetter IR, Musacchio A Cell. 2016 Nov 3;167(4):1028-1040.e15. doi: 10.1016/j.cell.2016.10.005. Epub 2016, Oct 27. PMID:27881301^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Obuse C, Iwasaki O, Kiyomitsu T, Goshima G, Toyoda Y, Yanagida M. A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1. Nat Cell Biol. 2004 Nov;6(11):1135-41. Epub 2004 Oct 24. PMID:15502821 doi:https://dx.doi.org/10.1038/ncb1187
↑ Kline SL, Cheeseman IM, Hori T, Fukagawa T, Desai A. The human Mis12 complex is required for kinetochore assembly and proper chromosome segregation. J Cell Biol. 2006 Apr 10;173(1):9-17. Epub 2006 Apr 3. PMID:16585270 doi:https://dx.doi.org/10.1083/jcb.200509158
↑ Petrovic A, Keller J, Liu Y, Overlack K, John J, Dimitrova YN, Jenni S, van Gerwen S, Stege P, Wohlgemuth S, Rombaut P, Herzog F, Harrison SC, Vetter IR, Musacchio A. Structure of the MIS12 Complex and Molecular Basis of Its Interaction with CENP-C at Human Kinetochores. Cell. 2016 Nov 3;167(4):1028-1040.e15. doi: 10.1016/j.cell.2016.10.005. Epub 2016, Oct 27. PMID:27881301 doi:https://dx.doi.org/10.1016/j.cell.2016.10.005

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OCA

[1] Obuse C, Iwasaki O, Kiyomitsu T, Goshima G, Toyoda Y, Yanagida M. A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1. Nat Cell Biol. 2004 Nov;6(11):1135-41. Epub 2004 Oct 24. PMID:15502821 doi:https://dx.doi.org/10.1038/ncb1187

[2] Kline SL, Cheeseman IM, Hori T, Fukagawa T, Desai A. The human Mis12 complex is required for kinetochore assembly and proper chromosome segregation. J Cell Biol. 2006 Apr 10;173(1):9-17. Epub 2006 Apr 3. PMID:16585270 doi:https://dx.doi.org/10.1083/jcb.200509158

[3] Petrovic A, Keller J, Liu Y, Overlack K, John J, Dimitrova YN, Jenni S, van Gerwen S, Stege P, Wohlgemuth S, Rombaut P, Herzog F, Harrison SC, Vetter IR, Musacchio A. Structure of the MIS12 Complex and Molecular Basis of Its Interaction with CENP-C at Human Kinetochores. Cell. 2016 Nov 3;167(4):1028-1040.e15. doi: 10.1016/j.cell.2016.10.005. Epub 2016, Oct 27. PMID:27881301 doi:https://dx.doi.org/10.1016/j.cell.2016.10.005

[1]

[2]

[3]

@@ Line 3: / Line 3: @@
 <StructureSection load='5lsi' size='340' side='right'caption='[[5lsi]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5lsi]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LSI OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5LSI FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5lsi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LSI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LSI FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.002&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">DSN1, C20orf172, MIS13 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), NSL1, C1orf48, DC31, DC8, MIS14 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5lsi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lsi OCA], [http://pdbe.org/5lsi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lsi RCSB], [http://www.ebi.ac.uk/pdbsum/5lsi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lsi ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5lsi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lsi OCA], [https://pdbe.org/5lsi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5lsi RCSB], [https://www.ebi.ac.uk/pdbsum/5lsi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5lsi ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/DSN1_HUMAN DSN1_HUMAN]] Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis.<ref>PMID:15502821</ref> <ref>PMID:16585270</ref>  [[http://www.uniprot.org/uniprot/NSL1_HUMAN NSL1_HUMAN]] Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis.<ref>PMID:16585270</ref>
+[https://www.uniprot.org/uniprot/DSN1_HUMAN DSN1_HUMAN] Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis.<ref>PMID:15502821</ref> <ref>PMID:16585270</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
 [[Category: Large Structures]]
-[[Category: Keller, J]]
+[[Category: Keller J]]
-[[Category: Liu, Y]]
+[[Category: Liu Y]]
-[[Category: Petrovic, A]]
+[[Category: Petrovic A]]
-[[Category: Vetter, I R]]
+[[Category: Vetter IR]]
-[[Category: Alpha-helical]]
-[[Category: Cell cycle]]

5lsi: Difference between revisions

Latest revision as of 18:45, 18 October 2023

CRYSTAL STRUCTURE OF THE KINETOCHORE MIS12 COMPLEX HEAD2 SUBDOMAIN CONTAINING DSN1 AND NSL1 FRAGMENTS

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

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