3nip: Difference between revisions

Latest revision as of 16:46, 1 November 2023

Crystal structure of Pseudomonas aeruginosa guanidinopropionase complexed with 1,6-diaminohexane

Structural highlights

3nip is a 6 chain structure with sequence from Pseudomonas aeruginosa PAO1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.5Å
Ligands:	16D
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GPUA_PSEAE Catalyzes the hydrolysis of 3-guanidinopropanoate to beta-alanine and urea. Possesses low activity against 4-guanidinobutanoate. Has no activity against arginine and agmatine.^[1]

Publication Abstract from PubMed

Pseudomonas aeruginosa guanidinobutyrase (GbuA) and guanidinopropionase (GpuA) catalyze the hydrolysis of 4-guanidinobutyrate and 3-guanidinopropionate, respectively. They belong to the ureohydrolase superfamily, which includes arginase, agmatinase, proclavaminate amidinohydrolase, and formiminoglutamase. In this study, we have determined the crystal structures of GbuA and GpuA from P. aeruginosa to provide a structural insight into their substrate specificity. Although GbuA and GpuA share a common structural fold of the typical ureohydrolase superfamily, they exhibit significant variations in two active site loops. Mutagenesis of Met161 of GbuA and Tyr157 of GpuA, both of which are located in the active site loop 1 and predicted to be involved in substrate recognition, significantly affected their enzymatic properties, implying their important roles in catalysis.

Crystal structures of Pseudomonas aeruginosa guanidinobutyrase and guanidinopropionase, members of the ureohydrolase superfamily.,Lee SJ, Kim DJ, Kim HS, Lee BI, Yoon HJ, Yoon JY, Kim KH, Jang JY, Im HN, An DR, Song JS, Kim HJ, Suh SW J Struct Biol. 2011 May 12. PMID:21600989^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Lee SJ, Kim DJ, Kim HS, Lee BI, Yoon HJ, Yoon JY, Kim KH, Jang JY, Im HN, An DR, Song JS, Kim HJ, Suh SW. Crystal structures of Pseudomonas aeruginosa guanidinobutyrase and guanidinopropionase, members of the ureohydrolase superfamily. J Struct Biol. 2011 May 12. PMID:21600989 doi:10.1016/j.jsb.2011.05.002
↑ Lee SJ, Kim DJ, Kim HS, Lee BI, Yoon HJ, Yoon JY, Kim KH, Jang JY, Im HN, An DR, Song JS, Kim HJ, Suh SW. Crystal structures of Pseudomonas aeruginosa guanidinobutyrase and guanidinopropionase, members of the ureohydrolase superfamily. J Struct Biol. 2011 May 12. PMID:21600989 doi:10.1016/j.jsb.2011.05.002

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Lee SJ, Kim DJ, Kim HS, Lee BI, Yoon HJ, Yoon JY, Kim KH, Jang JY, Im HN, An DR, Song JS, Kim HJ, Suh SW. Crystal structures of Pseudomonas aeruginosa guanidinobutyrase and guanidinopropionase, members of the ureohydrolase superfamily. J Struct Biol. 2011 May 12. PMID:21600989 doi:10.1016/j.jsb.2011.05.002

[2] Lee SJ, Kim DJ, Kim HS, Lee BI, Yoon HJ, Yoon JY, Kim KH, Jang JY, Im HN, An DR, Song JS, Kim HJ, Suh SW. Crystal structures of Pseudomonas aeruginosa guanidinobutyrase and guanidinopropionase, members of the ureohydrolase superfamily. J Struct Biol. 2011 May 12. PMID:21600989 doi:10.1016/j.jsb.2011.05.002

[1]

[2]

@@ Line 3: / Line 3: @@
 <StructureSection load='3nip' size='340' side='right'caption='[[3nip]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3nip]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NIP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NIP FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3nip]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NIP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NIP FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=16D:HEXANE-1,6-DIAMINE'>16D</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3nio|3nio]], [[3niq|3niq]]</div></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=16D:HEXANE-1,6-DIAMINE'>16D</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">gpuA, PA0288 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Guanidinopropionase Guanidinopropionase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.3.17 3.5.3.17] </span></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nip OCA], [https://pdbe.org/3nip PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nip RCSB], [https://www.ebi.ac.uk/pdbsum/3nip PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nip ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/GPUA_PSEAE GPUA_PSEAE] Catalyzes the hydrolysis of 3-guanidinopropanoate to beta-alanine and urea. Possesses low activity against 4-guanidinobutanoate. Has no activity against arginine and agmatine.<ref>PMID:21600989</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Guanidinopropionase]]
 [[Category: Large Structures]]
-[[Category: Pseae]]
+[[Category: Pseudomonas aeruginosa PAO1]]
-[[Category: An, D]]
+[[Category: An D]]
-[[Category: Im, H]]
+[[Category: Im H]]
-[[Category: Jang, J Y]]
+[[Category: Jang JY]]
-[[Category: Kim, D J]]
+[[Category: Kim DJ]]
-[[Category: Kim, H S]]
+[[Category: Kim HS]]
-[[Category: Kim, K H]]
+[[Category: Kim KH]]
-[[Category: Lee, S J]]
+[[Category: Lee SJ]]
-[[Category: Suh, S W]]
+[[Category: Suh SW]]
-[[Category: Yoon, H J]]
+[[Category: Yoon HJ]]
-[[Category: Yoon, J Y]]
+[[Category: Yoon JY]]
-[[Category: Gpua]]
-[[Category: Hydrolase]]
-[[Category: Pa0288]]

3nip: Difference between revisions

Latest revision as of 16:46, 1 November 2023

Crystal structure of Pseudomonas aeruginosa guanidinopropionase complexed with 1,6-diaminohexane

Structural highlights

Function

Publication Abstract from PubMed

References

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