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<StructureSection load='3qac' size='340' side='right'caption='[[3qac]], [[Resolution|resolution]] 2.27&Aring;' scene=''>
<StructureSection load='3qac' size='340' side='right'caption='[[3qac]], [[Resolution|resolution]] 2.27&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3qac]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Amahp Amahp]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QAC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QAC FirstGlance]. <br>
<table><tr><td colspan='2'>[[3qac]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Amaranthus_hypochondriacus Amaranthus hypochondriacus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QAC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QAC FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3kgl|3kgl]], [[3ksc|3ksc]], [[2e9q|2e9q]], [[2d5f|2d5f]], [[2d5h|2d5h]], [[1od5|1od5]], [[1fxz|1fxz]]</div></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.275&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qac FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qac OCA], [https://pdbe.org/3qac PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qac RCSB], [https://www.ebi.ac.uk/pdbsum/3qac PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qac ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qac FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qac OCA], [https://pdbe.org/3qac PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qac RCSB], [https://www.ebi.ac.uk/pdbsum/3qac PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qac ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/Q38712_AMAHP Q38712_AMAHP]
11S globulin is one of the major seed storage proteins in amaranth. Recombinant protein was produced as up to approximately 80% of the total bacterial protein using Escherichia coli Rosetta-gami (DE3) containing pET21d with amaranth 11S globulin cDNA. The best expression condition was at 302 K for 20 h using LB medium containing 0.5 M NaCl. The recombinant protein was easily separated from most of the Escherichia coli proteins by precipitation with 0-40% ammonium sulfate solution. It formed aggregates at low temperature and at low salt concentrations. This behaviour may imply that it has a more hydrophobic nature than other 11S seed globulins. The crystals diffracted to 6 A resolution and belonged to space group P6(3), with unit-cell parameters a=b=97.6, c=74.8 A, gamma=120.0 degrees. One subunit of a trimer was estimated to be present in the asymmetric unit, assuming a Vsol of 41%. To obtain the complete structure solution, experiments to improve crystallization and flash-cooling conditions are in progress.
 
Expression, purification and preliminary crystallization of amaranth 11S proglobulin seed storage protein from Amaranthus hypochondriacus L.,Tandang-Silvas MR, Carrazco-Pena L, Barba de la Rosa AP, Osuna-Castro JA, Utsumi S, Mikami B, Maruyama N Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Aug 1;66(Pt, 8):919-22. Epub 2010 Jul 29. PMID:20693668<ref>PMID:20693668</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3qac" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Amahp]]
[[Category: Amaranthus hypochondriacus]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Carrazco-Pena, L]]
[[Category: Barba de la Rosa AP]]
[[Category: Maruyama, N]]
[[Category: Carrazco-Pena L]]
[[Category: Mikami, B]]
[[Category: Maruyama N]]
[[Category: Osuna-Castro, J A]]
[[Category: Mikami B]]
[[Category: Rosa, A P.Barba de la]]
[[Category: Osuna-Castro JA]]
[[Category: Tandang-Silvas, M R]]
[[Category: Tandang-Silvas MR]]
[[Category: Utsumi, S]]
[[Category: Utsumi S]]
[[Category: Plant protein]]
[[Category: Seed storage protein]]

Revision as of 17:11, 1 November 2023

Structure of amaranth 11S proglobulin seed storage protein from Amaranthus hypochondriacus L.

3qac, resolution 2.27Å

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