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<StructureSection load='3sct' size='340' side='right'caption='[[3sct]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
<StructureSection load='3sct' size='340' side='right'caption='[[3sct]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3sct]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Japanese_rice Japanese rice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SCT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SCT FirstGlance]. <br>
<table><tr><td colspan='2'>[[3sct]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryza_sativa_Japonica_Group Oryza sativa Japonica Group]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SCT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SCT FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2rgl|2rgl]], [[2rgm|2rgm]], [[3f4v|3f4v]], [[3f5i|3f5i]], [[3f5j|3f5j]], [[3f5k|3f5k]], [[3aht|3aht]], [[3scn|3scn]], [[3sco|3sco]], [[3scp|3scp]], [[3scq|3scq]], [[3scr|3scr]], [[3scs|3scs]], [[3scu|3scu]], [[3scv|3scv]], [[3scw|3scw]]</div></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=PRD_900011:beta-cellotetraose'>PRD_900011</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BGLU1, BGLU7, LOC_Os03g49600, Os03g0703000, os3bglu7, OSJNBa0004L11.16 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=39947 Japanese rice])</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3sct FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sct OCA], [https://pdbe.org/3sct PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3sct RCSB], [https://www.ebi.ac.uk/pdbsum/3sct PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3sct ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3sct FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sct OCA], [https://pdbe.org/3sct PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3sct RCSB], [https://www.ebi.ac.uk/pdbsum/3sct PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3sct ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/BGL07_ORYSJ BGL07_ORYSJ]] Hydrolyzes p-nitrophenyl beta-D-glucoside, p-nitrophenyl beta-D-mannoside, p-nitrophenyl beta-D-galactoside, p-nitrophenyl beta-D-xyloside, p-nitrophenyl beta-D-fucoside, p-nitrophenyl beta-L-arabinoside, oligosaccharides, pyridoxine beta-D-glucoside and the cyanogenic glucosides amygdalin, prunasin and dhurrin. Possesses pyridoxine transglucosylation activity.<ref>PMID:14692878</ref> <ref>PMID:19766588</ref> <ref>PMID:18308333</ref>
[https://www.uniprot.org/uniprot/BGL07_ORYSJ BGL07_ORYSJ] Hydrolyzes p-nitrophenyl beta-D-glucoside, p-nitrophenyl beta-D-mannoside, p-nitrophenyl beta-D-galactoside, p-nitrophenyl beta-D-xyloside, p-nitrophenyl beta-D-fucoside, p-nitrophenyl beta-L-arabinoside, oligosaccharides, pyridoxine beta-D-glucoside and the cyanogenic glucosides amygdalin, prunasin and dhurrin. Possesses pyridoxine transglucosylation activity.<ref>PMID:14692878</ref> <ref>PMID:19766588</ref> <ref>PMID:18308333</ref>  


==See Also==
==See Also==
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Beta-glucosidase]]
[[Category: Japanese rice]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Cairns, J R.Ketudat]]
[[Category: Oryza sativa Japonica Group]]
[[Category: Kuaprasert, B]]
[[Category: Ketudat Cairns JR]]
[[Category: Pengthaisong, S]]
[[Category: Kuaprasert B]]
[[Category: Withers, S G]]
[[Category: Pengthaisong S]]
[[Category: Beta-alpha-barrel]]
[[Category: Withers SG]]
[[Category: Glycosynthase]]
[[Category: Hydrolase]]
[[Category: Oligosaccharide synthesis]]
[[Category: Transglucosylation]]

Revision as of 17:24, 1 November 2023

Crystal Structure of Rice BGlu1 E386G Mutant Complexed with Cellotetraose

3sct, resolution 1.60Å

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