3ujq

Galactose-specific lectin from Dolichos lablab in complex with galactose

spinqualitylabelsall models 3ujq, resolution 2.06Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3ujq is a 4 chain structure with sequence from Lablab purpureus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.06Å Ligands: CA, EDO, GAL, MN, PEG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function LECA2_LABPU D-galactose-binding lectin. Publication Abstract from PubMed Crystal structure analysis of a galactose-specific lectin from a leguminous food crop Dolichos lablab (Indian lablab beans) has been carried out to obtain insights into its quaternary association and lectin-carbohydrate interactions. The analysis led to the identification of adenine binding sites at the dimeric interfaces of the heterotetrameric lectin. Structural details of similar adenine binding were reported in only one legume lectin, Dolichos biflorus, before this study. Here, we present the structure of the galactose-binding D. lablab lectin at different pH values in the native form and in complex with galactose and adenine. This first structure report on this lectin also provides a high resolution atomic view of legume lectin-adenine interactions. The tetramer has two canonical and two DB58-like interfaces. The binding of adenine, a non-carbohydrate ligand, is found to occur at four hydrophobic sites at the core of the tetramer at the DB58-like dimeric interfaces and does not interfere with the carbohydrate-binding site. To support the crystallographic observations, the adenine binding was further quantified by carrying out isothermal calorimetric titration. By this method, we not only estimated the affinity of the lectin to adenine but also showed that adenine binds with negative cooperativity in solution. Affinity of a galactose-specific legume lectin from Dolichos lablab to adenine revealed by X-ray cystallography.,Shetty KN, Latha VL, Rao RN, Nadimpalli SK, Suguna K IUBMB Life. 2013 Jul;65(7):633-44. doi: 10.1002/iub.1177. PMID:23794513 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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