5mhj

ICP4 DNA-binding domain, lacking intrinsically disordered region, in complex with 12mer DNA duplex from its own promoter

spinqualitylabelsall models 5mhj, resolution 2.12Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5mhj is a 4 chain structure with sequence from Human alphaherpesvirus 1 and Human alphaherpesvirus 1 strain 17. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.117Å Ligands: ACY, CL Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function ICP4_HHV11 Plays an essential role in the regulation of viral gene expression by both activating and repressing host RNA polymerase II-mediated transcription. Binds with high affinity to the sequence 5'-ATCGTC-3'. Activates transcription by recruiting a form of the host TFIID to promoters and stabilizing the pre-initiation complex formation. Negatively regulates its own transcription. This immediate early (IE) protein is absolutely necessary for the transition from IE transcription to later viral gene transcription. Publication Abstract from PubMed The transcription factor ICP4 from herpes simplex virus has a central role in regulating the gene expression cascade which controls viral infection. Here we present the crystal structure of the functionally essential ICP4 DNA binding domain in complex with a segment from its own promoter, revealing a novel homo-dimeric fold. We also studied the complex in solution by small angle X-Ray scattering, nuclear magnetic resonance and surface-plasmon resonance which indicated that, in addition to the globular domain, a flanking intrinsically disordered region also recognizes DNA. Together the data provides a rationale for the bi-partite nature of the ICP4 DNA recognition consensus sequence as the globular and disordered regions bind synergistically to adjacent DNA motifs. Therefore in common with its eukaryotic host, the viral transcription factor ICP4 utilizes disordered regions to enhance the affinity and tune the specificity of DNA interactions in tandem with a globular domain. The herpes viral transcription factor ICP4 forms a novel DNA recognition complex.,Tunnicliffe RB, Lockhart-Cairns MP, Levy C, Mould AP, Jowitt TA, Sito H, Baldock C, Sandri-Goldin RM, Golovanov AP Nucleic Acids Res. 2017 May 13. doi: 10.1093/nar/gkx419. PMID:28505309 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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