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'''CRYSTAL STRUCTURE OF BACTERIOCIN AS-48 AT PH 7.5, PHOSPHATE BOUND. CRYSTAL FORM I''' | '''CRYSTAL STRUCTURE OF BACTERIOCIN AS-48 AT PH 7.5, PHOSPHATE BOUND. CRYSTAL FORM I''' | ||
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[[Category: Sanchez-Barrena, M J.]] | [[Category: Sanchez-Barrena, M J.]] | ||
[[Category: Valdivia, E.]] | [[Category: Valdivia, E.]] | ||
[[Category: | [[Category: Antibacterial peptide]] | ||
[[Category: | [[Category: Bacteriocin]] | ||
[[Category: | [[Category: Cyclic polypeptide]] | ||
[[Category: | [[Category: Membrane permeabilization]] | ||
[[Category: | [[Category: Protein crystallography]] | ||
[[Category: | [[Category: Protein membrane interaction]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 03:30:17 2008'' | |||
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Revision as of 00:30, 3 May 2008
CRYSTAL STRUCTURE OF BACTERIOCIN AS-48 AT PH 7.5, PHOSPHATE BOUND. CRYSTAL FORM I
Overview
The bacteriocin AS-48 is a membrane-interacting peptide, which displays a broad anti-microbial spectrum against Gram-positive and Gram-negative bacteria. The NMR structure of AS-48 at pH 3 has been solved. The analysis of this structure suggests that the mechanism of AS-48 anti-bacterial activity involves the accumulation of positively charged molecules at the membrane surface leading to a disruption of the membrane potential. Here, we report the high-resolution crystal structure of AS-48 and sedimentation equilibrium experiments showing that this bacteriocin is able to adopt different oligomeric structures according to the physicochemical environment. The analysis of these structures suggests a mechanism for molecular function of AS-48 involving a transition from a water-soluble form to a membrane-bound state upon membrane binding.
About this Structure
1O83 is a Single protein structure of sequence from Enterococcus faecalis. Full crystallographic information is available from OCA.
Reference
Structure of bacteriocin AS-48: from soluble state to membrane bound state., Sanchez-Barrena MJ, Martinez-Ripoll M, Galvez A, Valdivia E, Maqueda M, Cruz V, Albert A, J Mol Biol. 2003 Nov 28;334(3):541-9. PMID:14623193 Page seeded by OCA on Sat May 3 03:30:17 2008
