3n25

The structure of muscle pyruvate kinase in complex with proline, pyruvate, and Mn2+

spinqualitylabelsall models 3n25, resolution 2.41Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3n25 is a 8 chain structure with sequence from Oryctolagus cuniculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.41Å Ligands: EDO, GOL, K, MN, NA, PRO, PYR Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function KPYM_RABIT Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation (By similarity). Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed In the study of rabbit muscle pyruvate kinase (M(1)-PYK), proline has previously been used as an osmolyte in an attempt to determine a role for pre-existing conformational equilibria in allosteric regulation. In this context, osmolytes are small molecules assumed to have no direct interaction with the protein. In contrast to its proposed role as an osmolyte, the structure of M(1)PYK-Mn-pyruvate-proline complex reported herein demonstrates that proline binds specifically to the allosteric site of M(1)-PYK. Therefore, this amino acid is an allosteric effector, rather than a benign osmolyte. Other compounds often used as osmolytes (polyethyleneglycol and glycerol) are also present in the structure, suggesting an interaction with the protein that would, in turn, prevent the usefulness of these compounds in the study of this and most likely other proteins. These findings highlight the need to verify that compounds used as osmolytes to perturb pre-existing conformational equilibrium do not directly interact with the protein, a consideration not commonly addressed in the past. The pyruvate kinase model system, a cautionary tale for the use of osmolyte perturbations to support conformational equilibria in allostery.,Fenton AW, Johnson TA, Holyoak T Protein Sci. 2010 Jul 13. PMID:20629175 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Pyruvate kinase 3D structures

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