5xvq: Difference between revisions

Latest revision as of 08:15, 22 November 2023

Crystal structure of monkey Nicotinamide N-methyltransferase (NNMT) bound with end product, 1-methyl Nicotinamide (MNA)

Structural highlights

5xvq is a 2 chain structure with sequence from Macaca mulatta. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.29Å
Ligands:	8GC, GOL, SAH
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

F7ERX8_MACMU

Publication Abstract from PubMed

Nicotinamide N-methyltransferase (NNMT) is a S-adenosyl-l-methionine (SAM)-dependent enzyme that catalyzes N-methylation of nicotinamide (NA) and other pyridines to form N-methyl pyridinium ions. Here we report the first ternary complex X-ray crystal structures of monkey NNMT and mouse NNMT in bound form with the primary endogenous product, 1-methyl nicotinamide (MNA) and demethylated cofactor, S-adenosyl-homocysteine (SAH) determined at 2.30 A and 1.88 A respectively. The structural fold of these enzymes is identical to human NNMT. It is known that the primary endogenous product catalyzed by NNMT, MNA is a specific inhibitor of NNMT. Our data clearly indicates that the MNA binds to the active site and it would be trapped in the active site due to the formation of the bridge between the pole (long helix, alpha3) and long C-terminal loop. This might explain the mechanism of MNA acting as a feedback inhibitor of NNMT.

Crystal structures of monkey and mouse nicotinamide N-methyltransferase (NNMT) bound with end product, 1-methyl nicotinamide.,Swaminathan S, Birudukota S, Thakur MK, Parveen R, Kandan S, Juluri S, Shaik S, Anand NN, Burri RR, Kristam R, Hallur MS, Rajagopal S, Schreuder H, Langer T, Rudolph C, Ruf S, Dhakshinamoorthy S, Gosu R, Kannt A Biochem Biophys Res Commun. 2017 Jul 15. pii: S0006-291X(17)31437-7. doi:, 10.1016/j.bbrc.2017.07.087. PMID:28720493^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Swaminathan S, Birudukota S, Thakur MK, Parveen R, Kandan S, Juluri S, Shaik S, Anand NN, Burri RR, Kristam R, Hallur MS, Rajagopal S, Schreuder H, Langer T, Rudolph C, Ruf S, Dhakshinamoorthy S, Gosu R, Kannt A. Crystal structures of monkey and mouse nicotinamide N-methyltransferase (NNMT) bound with end product, 1-methyl nicotinamide. Biochem Biophys Res Commun. 2017 Jul 15. pii: S0006-291X(17)31437-7. doi:, 10.1016/j.bbrc.2017.07.087. PMID:28720493 doi:https://dx.doi.org/10.1016/j.bbrc.2017.07.087

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Swaminathan S, Birudukota S, Thakur MK, Parveen R, Kandan S, Juluri S, Shaik S, Anand NN, Burri RR, Kristam R, Hallur MS, Rajagopal S, Schreuder H, Langer T, Rudolph C, Ruf S, Dhakshinamoorthy S, Gosu R, Kannt A. Crystal structures of monkey and mouse nicotinamide N-methyltransferase (NNMT) bound with end product, 1-methyl nicotinamide. Biochem Biophys Res Commun. 2017 Jul 15. pii: S0006-291X(17)31437-7. doi:, 10.1016/j.bbrc.2017.07.087. PMID:28720493 doi:https://dx.doi.org/10.1016/j.bbrc.2017.07.087

[1]

@@ Line 1: / Line 1: @@
 ==Crystal structure of monkey Nicotinamide N-methyltransferase (NNMT) bound with end product, 1-methyl Nicotinamide (MNA)==
-<StructureSection load='5xvq' size='340' side='right' caption='[[5xvq]], [[Resolution|resolution]] 2.29&Aring;' scene=''>
+<StructureSection load='5xvq' size='340' side='right'caption='[[5xvq]], [[Resolution|resolution]] 2.29&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5xvq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Macmu Macmu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XVQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XVQ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5xvq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Macaca_mulatta Macaca mulatta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XVQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XVQ FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=8GC:1-methylpyridine-3-carboxamide'>8GC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.29&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NNMT, EGK_06915 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9544 MACMU])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=8GC:3-carbamoyl-1-methylpyridin-1-ium'>8GC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xvq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xvq OCA], [http://pdbe.org/5xvq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xvq RCSB], [http://www.ebi.ac.uk/pdbsum/5xvq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xvq ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xvq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xvq OCA], [https://pdbe.org/5xvq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xvq RCSB], [https://www.ebi.ac.uk/pdbsum/5xvq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xvq ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/F7ERX8_MACMU F7ERX8_MACMU]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Macmu]]
+[[Category: Large Structures]]
-[[Category: Birudukota, S]]
+[[Category: Macaca mulatta]]
-[[Category: Gosu, R]]
+[[Category: Birudukota S]]
-[[Category: Kandan, S]]
+[[Category: Gosu R]]
-[[Category: Kannt, A]]
+[[Category: Kandan S]]
-[[Category: Parveen, R]]
+[[Category: Kannt A]]
-[[Category: Swaminathan, S]]
+[[Category: Parveen R]]
-[[Category: Thakur, M K]]
+[[Category: Swaminathan S]]
-[[Category: Feedback inhibition]]
+[[Category: Thakur MK]]
-[[Category: Mna]]
-[[Category: Nnmt]]
-[[Category: T2d]]
-[[Category: Ternary complex]]
-[[Category: Transferase]]

5xvq: Difference between revisions

Latest revision as of 08:15, 22 November 2023

Crystal structure of monkey Nicotinamide N-methyltransferase (NNMT) bound with end product, 1-methyl Nicotinamide (MNA)

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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