5zw3

Crystal Structure of TrmR from B. subtilis

spinqualitylabelsall models 5zw3, resolution 2.27Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5zw3 is a 2 chain structure with sequence from Bacillus subtilis subsp. subtilis str. 168. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.27Å Ligands: MG, SAH Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function TRMR_BACSU Catalyzes the methylation of 5-hydroxyuridine (ho5U) to form 5-methoxyuridine (mo5U) at position 34 in tRNAs.[HAMAP-Rule:MF_02217] Publication Abstract from PubMed Derivatives of 5-hydroxyuridine (ho5U), such as 5-methoxyuridine (mo5U) and 5-oxyacetyluridine (cmo5U), are ubiquitous modifications of the wobble position of bacterial tRNA that are believed to enhance translational fidelity by the ribosome. In gram-negative bacteria, the last step in the biosynthesis of cmo5U from ho5U involves the unique metabolite carboxy S-adenosylmethionine (Cx-SAM) and the carboxymethyl transferase CmoB. However, the equivalent position in the tRNA of Gram-positive bacteria is instead mo5U, where the methyl group is derived from SAM and installed by an unknown methyltransferase. By utilizing a cmoB-deficient strain of Escherichia coli as a host and assaying for the formation of mo5U in total RNA isolates with methyltransferases of unknown function from Bacillus subtilis, we found that this modification is installed by the enzyme TrmR (formerly known as YrrM). Furthermore, X-ray crystal structures of TrmR with and without the anticodon stemloop of tRNAAla have been determined, which provide insight into both sequence and structure specificity in the interactions of TrmR with tRNA. Identification of a novel tRNA wobble uridine modifying activity in the biosynthesis of 5-methoxyuridine.,Ryu H, Grove TL, Almo SC, Kim J Nucleic Acids Res. 2018 Jun 30. pii: 5047278. doi: 10.1093/nar/gky592. PMID:29982645 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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