6a5h

The structure of [4+2] and [6+4] cyclase in the biosynthetic pathway of unidentified natural product

spinqualitylabelsall models 6a5h, resolution 1.62Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6a5h is a 2 chain structure with sequence from Nocardia tenerifensis NBRC 101015. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.618Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Pericyclic reactions are powerful transformations for the construction of carbon-carbon and carbon-heteroatom bonds in organic synthesis. Their role in biosynthesis is increasingly apparent, and mechanisms by which pericyclases can catalyse reactions are of major interest(1). [4+2] cycloadditions (Diels-Alder reactions) have been widely used in organic synthesis(2) for the formation of six-membered rings and are now well-established in biosynthesis(3-6). [6+4] and other 'higher-order' cycloadditions were predicted(7) in 1965, and are now increasingly common in the laboratory despite challenges arising from the generation of a highly strained ten-membered ring system(8,9). However, although enzyme-catalysed [6+4] cycloadditions have been proposed(10-12), they have not been proven to occur. Here we demonstrate a group of enzymes that catalyse a pericyclic [6+4] cycloaddition, which is a crucial step in the biosynthesis of streptoseomycin-type natural products. This type of pericyclase catalyses [6+4] and [4+2] cycloadditions through a single ambimodal transition state, which is consistent with previous proposals(11,12). The [6+4] product is transformed to a less stable [4+2] adduct via a facile Cope rearrangement, and the [4+2] adduct is converted into the natural product enzymatically. Crystal structures of three pericyclases, computational simulations of potential energies and molecular dynamics, and site-directed mutagenesis establish the mechanism of this transformation. This work shows how enzymes are able to catalyse concerted pericyclic reactions involving ambimodal transition states. Enzyme-catalysed [6+4] cycloadditions in the biosynthesis of natural products.,Zhang B, Wang KB, Wang W, Wang X, Liu F, Zhu J, Shi J, Li LY, Han H, Xu K, Qiao HY, Zhang X, Jiao RH, Houk KN, Liang Y, Tan RX, Ge HM Nature. 2019 Apr;568(7750):122-126. doi: 10.1038/s41586-019-1021-x. Epub 2019 Mar, 13. PMID:30867595 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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