6ihx

Crystal Structure Analysis of bovine Hemoglobin modified by SNP

spinqualitylabelsall models 6ihx, resolution 1.46Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6ihx is a 4 chain structure with sequence from Bos taurus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.46Å Ligands: CMO, HEM Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function HBA_BOVIN Involved in oxygen transport from the lung to the various peripheral tissues. Publication Abstract from PubMed A core-shell ensemble of bovine hemoglobin (Hb) and human serum albumin (HSA) is an artificial O2 carrier as a red blood cell substitute. This protein particle is created by covalent wrapping of a carbonyl Hb with HSAs: HbR-HSA3 cluster, where HbR signifies the use of carbonyl Hb [relaxed(R)-state conformation] as a starting material. The HbR-HSA3 cluster exhibits high O2 affinity and low cooperativity. Analysis of the quaternary structure of the central HbR in the cluster revealed that its high O2 affinity is attributed to the physically immobile HbR nucleus. Circular dichroism and UV-vis absorption spectroscopy showed that the structure of deoxy HbR core closely resembles the R-state. The crystal structure of Lys-modified carbonyl HbR was superimposed to that of carbonyl Hb. These results imply that chemical modifications of the surface Lys groups and Cys-93(beta) of the carbonyl Hb with crosslinking agent interfered in the quaternary structure movement from the R-state to Tense(T)-state. As expected, coupling of deoxy Hb (T-state) with HSAs yielded HbT-HSA3 cluster having low O2 affinity. The mixing of HbR-HSA3 and HbT-HSA3 clusters conferred a tailor-made formulation of artificial O2 carrier with a desired O2 affinity (P50). Quaternary Structure Analysis of a Hemoglobin Core in Hemoglobin-Albumin Cluster.,Morita Y, Yamada T, Kureishi M, Kihira K, Komatsu T J Phys Chem B. 2018 Nov 16. doi: 10.1021/acs.jpcb.8b10077. PMID:30444368 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Hemoglobin 3D structures

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