5nuz: Difference between revisions

Latest revision as of 12:09, 22 November 2023

Junin virus GP1 glycoprotein in complex with an antibody Fab fragment

Structural highlights

5nuz is a 6 chain structure with sequence from Argentinian mammarenavirus and Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.85Å
Ligands:	BMA, GOL, IPA, MAN, NAG
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

C1K9J9_JUNIN

Publication Abstract from PubMed

Transmission of hemorrhagic fever New World arenaviruses from their rodent reservoirs to human populations poses substantial public health and economic dangers. These zoonotic events are enabled by the specific interaction between the New World arenaviral attachment glycoprotein, GP1, and cell surface human transferrin receptor (hTfR1). Here, we present the structural basis for how a mouse-derived neutralizing antibody (nAb), OD01, disrupts this interaction by targeting the receptor-binding surface of the GP1 glycoprotein from Junin virus (JUNV), a hemorrhagic fever arenavirus endemic in central Argentina. Comparison of our structure with that of a previously reported nAb complex (JUNV GP1-GD01) reveals largely overlapping epitopes but highly distinct antibody-binding modes. Despite differences in GP1 recognition, we find that both antibodies present a key tyrosine residue, albeit on different chains, that inserts into a central pocket on JUNV GP1 and effectively mimics the contacts made by the host TfR1. These data provide a molecular-level description of how antibodies derived from different germline origins arrive at equivalent immunological solutions to virus neutralization.

Convergent immunological solutions to Argentine hemorrhagic fever virus neutralization.,Zeltina A, Krumm SA, Sahin M, Struwe WB, Harlos K, Nunberg JH, Crispin M, Pinschewer DD, Doores KJ, Bowden TA Proc Natl Acad Sci U S A. 2017 Jul 3;114(27):7031-7036. doi:, 10.1073/pnas.1702127114. Epub 2017 Jun 19. PMID:28630325^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Zeltina A, Krumm SA, Sahin M, Struwe WB, Harlos K, Nunberg JH, Crispin M, Pinschewer DD, Doores KJ, Bowden TA. Convergent immunological solutions to Argentine hemorrhagic fever virus neutralization. Proc Natl Acad Sci U S A. 2017 Jul 3;114(27):7031-7036. doi:, 10.1073/pnas.1702127114. Epub 2017 Jun 19. PMID:28630325 doi:https://dx.doi.org/10.1073/pnas.1702127114

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Zeltina A, Krumm SA, Sahin M, Struwe WB, Harlos K, Nunberg JH, Crispin M, Pinschewer DD, Doores KJ, Bowden TA. Convergent immunological solutions to Argentine hemorrhagic fever virus neutralization. Proc Natl Acad Sci U S A. 2017 Jul 3;114(27):7031-7036. doi:, 10.1073/pnas.1702127114. Epub 2017 Jun 19. PMID:28630325 doi:https://dx.doi.org/10.1073/pnas.1702127114

[1]

@@ Line 1: / Line 1: @@
 ==Junin virus GP1 glycoprotein in complex with an antibody Fab fragment==
-<StructureSection load='5nuz' size='340' side='right' caption='[[5nuz]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
+<StructureSection load='5nuz' size='340' side='right'caption='[[5nuz]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5nuz]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Junin Junin] and [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NUZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NUZ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5nuz]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Argentinian_mammarenavirus Argentinian mammarenavirus] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NUZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NUZ FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GPC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11619 JUNIN])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nuz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nuz OCA], [http://pdbe.org/5nuz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nuz RCSB], [http://www.ebi.ac.uk/pdbsum/5nuz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nuz ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5nuz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nuz OCA], [https://pdbe.org/5nuz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5nuz RCSB], [https://www.ebi.ac.uk/pdbsum/5nuz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5nuz ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/C1K9J9_JUNIN C1K9J9_JUNIN]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 17: / Line 19: @@
 </div>
 <div class="pdbe-citations 5nuz" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Glycoprotein GP 3D structures|Glycoprotein GP 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Junin]]
+[[Category: Argentinian mammarenavirus]]
-[[Category: Lk3 transgenic mice]]
+[[Category: Large Structures]]
-[[Category: Bowden, T A]]
+[[Category: Mus musculus]]
-[[Category: Crispin, M]]
+[[Category: Bowden TA]]
-[[Category: Doores, K J]]
+[[Category: Crispin M]]
-[[Category: Harlos, K]]
+[[Category: Doores KJ]]
-[[Category: Krumm, S A]]
+[[Category: Harlos K]]
-[[Category: Nunberg, J H]]
+[[Category: Krumm SA]]
-[[Category: Pinschewer, D D]]
+[[Category: Nunberg JH]]
-[[Category: Sahin, M]]
+[[Category: Pinschewer DD]]
-[[Category: Struwe, W B]]
+[[Category: Sahin M]]
-[[Category: Zeltina, A]]
+[[Category: Struwe WB]]
-[[Category: Immune system]]
+[[Category: Zeltina A]]
-[[Category: Viral antigen-antibody complex]]

5nuz: Difference between revisions

Latest revision as of 12:09, 22 November 2023

Junin virus GP1 glycoprotein in complex with an antibody Fab fragment

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

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